%0 Journal Article
%A Altea-Manzano, Patricia
%A Unciti-Broceta, Juan Diego
%A Cano-Cortes, Victoria
%A Ruiz-Blas, María Paz
%A Valero-Griñan, Teresa
%A Diaz-Mochon, Juan Jose
%A Sanchez-Martin, Rosario
%T Tracking cell proliferation using a nanotechnology-based approach.
%D 2017
%U http://hdl.handle.net/10668/11202
%X To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. [Formula: see text].
%K cell proliferation
%K cell tracking
%K flow cytometry
%K fluorescent nanoparticles
%K nanofection
%~