RT Journal Article
T1 The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray
A1 Palacín, Arantxa
A1 Rivas, Luis A.
A1 Gómez-Casado, Cristina
A1 Aguirre, Jacobo
A1 Tordesillas, Leticia
A1 Bartra, Joan
A1 Blanco, Carlos
A1 Carrillo, Teresa
A1 Cuesta-Herranz, Javier
A1 Cumplido Bonny, José A.
A1 Flores, Enrique
A1 García-Alvarez-Eire, Mar G.
A1 García-Nuñez, Ignacio
A1 Fernández, Francisco J.
A1 Gamboa, Pedro
A1 Muñoz, Rosa
A1 Sánchez-Monge, Rosa
A1 Torres, Maria
A1 Varela Losada, Susana
A1 Villalba, Mayte
A1 Vega, Francisco
A1 Parro, Victor
A1 Blanca, Miguel
A1 Salcedo, Gabriel
A1 Díaz-Perales, Araceli
K1 España
K1 Geografía
K1 Reacciones cruzadas
K1 Hipersensibilidad a los alimentos
K1 Inmunización
K1 Inmunoensayo
K1 Inmunoglobulina E
K1 Peso molecular
K1 Proteínas de plantas
K1 Polen
K1 Análisis por matrices de proteínas
AB Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.
PB Public Library of Science
YR 2012
FD 2012-09-07
LK http://hdl.handle.net/10668/1409
UL http://hdl.handle.net/10668/1409
LA en
NO Palacín A, Rivas LA, Gómez-Casado C, Aguirre J, Tordesillas L, Bartra J, et al. The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray. PLoS ONE. 2012; 7(9):e44088
NO Multicenter Study; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 10, 2025