RT Journal Article
T1 Three Fusarium oxysporum mitogen-activated protein kinases (MAPKs) have distinct and complementary roles in stress adaptation and cross-kingdom pathogenicity.
A1 Segorbe, David
A1 Di Pietro, Antonio
A1 Perez-Nadales, Elena
A1 Turra, David
K1 Fusarium oxysporum
K1 MAPKs
K1 Cross-talk
K1 Development
K1 Stress response
K1 Virulence
AB Mitogen-activated protein kinase (MAPK) cascades mediate cellular responses to environmental signals. Previous studies in the fungal pathogen Fusarium oxysporum have revealed a crucial role of Fmk1, the MAPK orthologous to Saccharomyces cerevisiae Fus3/Kss1, in vegetative hyphal fusion and plant infection. Here, we genetically dissected the individual and combined contributions of the three MAPKs Fmk1, Mpk1 and Hog1 in the regulation of development, stress response and virulence of F. oxysporum on plant and animal hosts. Mutants lacking Fmk1 or Mpk1 were affected in reactive oxygen species (ROS) homeostasis and impaired in hyphal fusion and aggregation. Loss of Mpk1 also led to increased sensitivity to cell wall and heat stress, which was exacerbated by simultaneous inactivation of Fmk1, suggesting that both MAPKs contribute to cellular adaptation to high temperature, a prerequisite for mammalian pathogens. Deletion of Hog1 caused increased sensitivity to hyperosmotic stress and resulted in partial rescue of the restricted colony growth phenotype of the mpk1Δ mutant. Infection assays on tomato plants and the invertebrate animal host Galleria mellonella revealed distinct and additive contributions of the different MAPKs to virulence. Our results indicate that positive and negative cross-talk between the three MAPK pathways regulates stress adaptation, development and virulence in the cross-kingdom pathogen F. oxysporum.
PB Wiley
YR 2016
FD 2016-09-29
LK http://hdl.handle.net/10668/10178
UL http://hdl.handle.net/10668/10178
LA en
NO Segorbe D, Di Pietro A, Pérez-Nadales E, Turrà D. Three Fusarium oxysporum mitogen-activated protein kinases (MAPKs) have distinct and complementary roles in stress adaptation and cross-kingdom pathogenicity. Mol Plant Pathol. 2017 Sep;18(7):912-924
NO The authors are grateful to E. Martınez Aguilera for technical assistance.This work was supported by grants BIO2010-15505 and BIO2013-47870-R from the Spanish Ministerio de Innovacion y Competitividad (MINECO),BIO-296 from Junta de Andalucia and BIO2008-04479 from MINECO/ERA-NET PathoGenoMics to A.D.P
DS RISalud
RD Apr 8, 2025