RT Journal Article
T1 Arthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesis.
A1 Amadeu, Simone Oliveira
A1 Sarmiento-Machado, Luis Manuel
A1 Bartolomeu, Ariane Rocha
A1 Chaves, Maria Angel Garcia
A1 Romualdo, Guilherme Ribeiro
A1 de Moura, Nelci Antunes
A1 Barbisan, Luis Fernando
K1 Arthrospira (Spirulina) platensis
K1 Colon cancer prevention
K1 Colonic preneoplastic lesions
K1 Notch and DNA repair genes
K1 Tumour initiation
AB Colorectal cancer is the third most diagnosed cancer worldwide and linked to dietary/lifestyle factors. Arthrospira (Spirulina) platensis (AP) contains bioactive compounds with beneficial effects in vivo/in vitro. We evaluated the effects of AP feeding against 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Male Sprague Dawley rats were given subcutaneous injections of DMH (4 × 40 mg/kg body weight) (G1-G3) or vehicle (G4-G5) twice a week (weeks 3-4). During weeks 1-4, animals were fed a diet containing 1 % (G2) or 2 % (G3-G4) AP powder (w/w). After this period, all groups received a balanced diet until week 12. Some animals were euthanised after the last DMH injection (week 4) for histological, immunohistochemical (Ki-67, γ-H2AX and caspase-3) and molecular analyses (real time-PCR for 91 genes), while other animals were euthanised at week 12 for preneoplastic aberrant crypt foci (ACF) analysis. Both AP treatments (G2-G3) significantly decreased the DMH-induced increase in γ-H2AX (DNA damage) and caspase 3 (DNA damage-induced cell death) in colonic crypts at week 4. In addition, Cyp2e1 (Drug metabolism), Notch1, Notch2 and Jag1 genes (Notch pathway) and Atm, Wee1, Chek2, Mgmt, Ogg1 and Xrcc6 genes (DNA repair) were also down-regulated by 2 % AP feeding (G3) at week 4. A significant reduction in ACF development was observed in both AP-treated groups (G2-G3) at week 12. In conclusion, findings indicate that AP feeding reduced acute colonic damage after DMH, resulting in fewer preneoplastic lesions. Our study provided mechanistic insights on dietary AP-preventive effects against early colon carcinogenesis.
PB Frontiers Research Foundation
YR 2022
FD 2022-04-22
LK http://hdl.handle.net/10668/22570
UL http://hdl.handle.net/10668/22570
LA en
NO Amadeu SO, Sarmiento-Machado LM, Bartolomeu AR, Chaves MAG, Romualdo GR, de Moura NA, et al. Arthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesis. Br J Nutr. 2023 Feb 14;129(3):395-405.
DS RISalud
RD Apr 7, 2025