RT Journal Article
T1 Modulation of Monocyte Activation and Function during Direct Antiviral Agent Treatment in Patients Coinfected with HIV and Hepatitis C Virus.
A1 De Pablo-Bernal, Rebeca S
A1 Jimenez-Leon, M Reyes
A1 Tarancon-Diez, Laura
A1 Gutierrez-Valencia, Alicia
A1 Serna-Gallego, Ana
A1 Trujillo-Rodriguez, Maria
A1 Alvarez-Rios, Ana I
A1 Milanes-Guisado, Yusnelkis
A1 Espinosa, Nuria
A1 Roca-Oporto, Cristina
A1 Viciana, Pompeyo
A1 Lopez-Cortes, Luis F
A1 Ruiz-Mateos, Ezequiel
K1 DAAs
K1 HCV
K1 HIV
K1 activation
K1 cell-associated DNA
K1 inflammation
K1 monocyte
K1 polyfunctionality
K1 reservoir
AB The activation phenotypes and functional changes in monocyte subsets during hepatitis C virus (HCV) elimination in HIV/HCV-coinfected patients were evaluated. Twenty-two HIV/HCV-coinfected patients on suppressive combination antiretroviral treatment (cART) achieving HCV elimination after direct-acting antiviral (DAA) therapy and 10 HIV-monoinfected patients were included. The activation phenotype (10 markers) and polyfunctionality (intracellular interleukin-1α [IL-1α], IL-1β, IL-6, IL-8, tumor necrosis factor alpha [TNF-α], and IL-10 production) in three monocyte subsets (classical, intermediate, and nonclassical) were evaluated by flow cytometry before and at the end of treatment. Cell-associated HIV DNA levels were assayed by droplet digital PCR. After HCV clearance, there was a significant increase in classical monocyte and decreases in intermediate and nonclassical monocyte levels. The levels of the activation markers CD49d, CD40, and CX3CR1 were decreased after treatment in the monocyte subsets, reaching the levels in HIV-monoinfected patients. After lipopolysaccharide (LPS) stimulation, although polyfunctionality significantly decreased in intermediate and nonclassical monocytes, some combinations, such as the IL-1α- (IL-1α-negative) IL-1β- IL-6+ (IL-6-producing) IL-8- TNF-α- IL-10- combination, were remarkably increased at the end of treatment compared to the control group. Cell-associated HIV DNA levels correlated with activation markers before but not after treatment. HCV clearance after DAA treatment in patients on cART exerts an anti-inflammatory profile on monocyte subsets, activation phenotypes, and polyfunctionality. However, there is not a complete normalization compared with HIV-monoinfected patients.
YR 2020
FD 2020-08-20
LK http://hdl.handle.net/10668/15797
UL http://hdl.handle.net/10668/15797
LA en
DS RISalud
RD Mar 13, 2025