RT Journal Article
T1 GPETAFLR, a novel bioactive peptide from Lupinus angustifolius L. protein hydrolysate, reduces osteoclastogenesis
A1 Milian-Linares, M. Carmen
A1 Lemus-Conejo, Ana
A1 Mar-Yust, M.
A1 Pedroche, Justo
A1 Carrillo-Vico, Antonio
A1 Milian, Francisco
A1 Montserrat-de-la-Paz, Sergio
K1 Peptide
K1 Protein hydrolysate
K1 Lupine
K1 Osteoclast
K1 Osteoporosis
K1 Bone
K1 Osteoporosis
K1 Inhibition
K1 Rankl/opg
K1 Pathway
AB The effect of GPETAFLR, a peptide isolated from Lupinus angustifolius L. protein hydrolysate (LPH), on osteoclastogenesis was investigated. Human osteoclasts generated from monocytes were used to analyse the effects of GPETAFLR (50-100 mu g/mL) on osteoclastogenesis using TRAP reaction, RT-qPCR, and ELISA procedures. LPS enhanced TRAP activity and the expression of osteoclast marker genes (TRAP, OSCAR, RANK, and CATHK) while downregulated the expression of OPG gene in human monocyte-derived osteoclasts. These effects were reduced with GPETAFLR. Moreover, LPS increased the release of osteoclastogenic cytokines (TNF-alpha, IL-1 beta and IL-6) meanwhile GPETAFLR increased the release of anti-osteoclastogenic cytokines (IL-4 and IL-10) in the medium of human monocyte-derived osteoclasts. For the first time, we show that plant peptides from lupine protein hydrolysates have anti-osteoclastogenic activity. These exciting findings open opportunities for developing nutritional strategies with Lupinus angustifolius L. as dietary source of plant proteins, notably GPETAFLR, to prevent development and progression of osteoclast-related diseases.
PB Elsevier science bv
SN 1756-4646
YR 2018
FD 2018-06-08
LK http://hdl.handle.net/10668/18818
UL http://hdl.handle.net/10668/18818
LA en
NO Millan-Linares M.C.,  Lemus-Conejo A, Mar Yust M, Pedroche J, Carrillo-Vico A, Millan F, et al. GPETAFLR, a novel bioactive peptide from Lupinus angustifolius L. protein hydrolysate, reduces osteoclastogenesis. J. funct. food. 2018-Jun-08 (47) 299-303
DS RISalud
RD Jul 6, 2025