RT Journal Article
T1 Stent-induced tracheal stenosis can be predicted by IL-8 expression in rabbits.
A1 Arellano-Orden, Elena
A1 Serrano, Carolina
A1 Montes-Worboys, Ana
A1 Sánchez-López, Verónica
A1 Laborda, Alicia
A1 Lostalé, Fernando
A1 Lahuerta, Celia
A1 Rodríguez-Panadero, Francisco
A1 de Gregorio, Miguel Ángel
K1 Cytokine
K1 fibrosis
K1 rabbits
K1 stents
K1 tracheal stenosis
AB Bare metal stents may cause complications like fibrous encapsulation, granulation and tracheal stenosis. We investigated the behaviour of three commercially available stents in vivo (rabbits) and in vitro (coculture of those stents with epithelial and fibroblast cell lines). Also, we investigated whether development of tracheal stenosis could be predicted by any biological marker. The tracheae of 30 rabbits were implanted with either nitinol stents, with or without paclitaxel elution, or a cobalt-based stent. An additional ten rabbits underwent mock implantation (controls). Serial peripheral venous blood samples were taken throughout the study, and several cytokines measured. Animals were euthanized on day 90, with immediate tracheal endoscopy and lavage performed, then necropsy. Rabbits with cobalt-based stent exhibited more inflammation and the highest stenosis incidence, with reduced survival. Both in vivo and in vitro, this stent induced higher IL-8 levels than nitinol stents. Most important, the presence of stent-induced tracheal stenosis was closely associated to increase in IL-8 expression in blood just 1 day after tracheal stent implantation: a 1·19-fold increase vs. baseline had 83% sensitivity, 83% specificity, 77% positive predictive value, 88% negative predictive value and 83% accuracy to predict development of stenosis. The cobalt-based stent had the highest incidence of tracheal inflammation and stenosis. On the other hand, the paclitaxel-eluting nitinol stent did not prevent those complications and provoked a marked reaction compared with the bare nitinol stent. Early increase in IL-8 expression in blood after stent implantation could predict development of tracheal stenosis in rabbits.
YR 2016
FD 2016-12-20
LK http://hdl.handle.net/10668/10627
UL http://hdl.handle.net/10668/10627
LA en
DS RISalud
RD Apr 7, 2025