%0 Journal Article
%A Díaz-Santiago, Elena
%A Rodríguez-Caso, Luis
%A Cárdenas, Casimiro
%A Serrano, José J
%A Quesada, Ana R
%A Medina, Miguel Ángel
%T Homocysteine pre-treatment increases redox capacity in both endothelial and tumor cells.
%D 2016
%U http://hdl.handle.net/10668/10106
%X We studied the modulatory effects of homocysteine pre-treatment on the disulfide reduction capacity of tumor and endothelial cells. Human MDA-MB-231 breast carcinoma and bovine aorta endothelial cells were pre-treated for 1-24 hours with 0.5-5 mM homocysteine or homocysteine thiolactone. After washing to eliminate any rest of homocysteine or homocysteine thiolactone, cell redox capacity was determined by using a method for measuring disulfide reduction. Homocysteine pre-treatments for 1-4 hours at a concentration of 0.5-5 mM increase the disulfide reduction capacity of both tumor and endothelial cells. This effect cannot be fully mimicked by either cysteine or homocysteine thiolactone pre-treatments of tumor cells. Taken together, our data suggest that homocysteine can behave as an anti-oxidant agent by increasing the anti-oxidant capacity of tumor and endothelial cells.
%K Bovine aortic endothelial cells
%K Cysteine
%K Homocysteine
%K Homocysteine thiolactone
%K MDA-MB231 breast cancer cell
%K Redox
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