RT Journal Article
T1 Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression
A1 Pasquier, Jeremy
A1 Lafont, Anne-Gaelle
A1 Denis, Florian
A1 Lefranc, Benjamin
A1 Dubessy, Christophe
A1 Moreno-Herrera, Antonio
A1 Vaudry, Hubert
A1 Leprince, Jerome
A1 Dufour, Sylvie
A1 Rousseau, Karine
K1 Kisspeptins
K1 Tissue distribution
K1 Functional activity
K1 Pituitary cell culture
K1 LH
K1 GnRH receptor
K1 European eel
AB The European eel (Anguilla anguilla) presents a blockade of sexual maturation at a prepubertal stage due to a deficient production of gonadotropins. We previously initiated, in the eel, the investigation of the kisspeptin system, one of the major gatekeepers of puberty in mammals, and we predicted the sequence of two Kiss genes. In the present study, we cloned and sequenced Kiss1 and Kiss2 cDNAs from the eel brain. The tissue distributions of Kiss1 and Kiss2 transcripts, as investigated by quantitative real-time PCR, showed that both genes are primarily expressed in the eel brain and pituitary. The two 10-residue long sequences characteristic of kisspeptin, eel Kp1(10) and Kp2(10), as well as two longer sequences, predicted as mature peptides, eel Kp1(15) and Kp2(12), were synthesized and functionally analyzed. Using rat Kiss1 receptor-transfected Chinese hamster ovary cells, we found that the four synthesized eel peptides were able to induce [Ca2+](i) responses, indicating their ability to bind mammalian KissR-1 and to activate second messenger pathways. In primary culture of eel pituitary cells, all four peptides were able to specifically and dose-dependently inhibit lh beta expression, without any effect on fsh beta, confirming our previous data with heterologous kisspeptins. Furthermore, in this eel in vitro system, all four peptides inhibited the expression of the type 2 GnRH receptor (gnrh-r2). Our data revealed a dual inhibitory effect of homologous kisspeptins on both pituitary lh beta and gnrh-r2 expression in the European eel.
PB Frontiers Research Foundation
SN 1664-2392
YR 2017
FD 2017-12-04
LK http://hdl.handle.net/10668/19217
UL http://hdl.handle.net/10668/19217
LA en
NO Pasquier J, Lafont AG, Denis F, Lefranc B, Dubessy C, Moreno-Herrera A, et al.  Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression. Front Endocrinol (Lausanne). 2018 Jan 8;8:353
NO We thank Eric Ryckelynck and his team from Nodaiwa (Paris, France) for their kind cooperation. This work was supported by grants from the European Community PRO-EEL No. 245257 to A-GL and SD, from the National Research Agency NEMO No. ANR-08-BLAN-0173 to JP, A-GL, KR, SD, BL, and JL and from FrenchKiSS No. ANR-07-BLAN-0056-04 to JL and HV.
DS RISalud
RD Apr 18, 2025