RT Journal Article
T1 Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes.
A1 Moyá, Maria Luisa
A1 López-López, Manuel
A1 Lebrón, José Antonio
A1 Ostos, Francisco José
A1 Pérez, David
A1 Camacho, Vanesa
A1 Beck, Irene
A1 Merino-Bohórquez, Vicente
A1 Camean, Manuel
A1 Madinabeitia, Nuria
A1 López-Cornejo, Pilar
K1 bactericidal activity
K1 cefepime
K1 encapsulation
K1 liposome
K1 surfactant.
K1 zeta potential
AB Cefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic has several side effects and a high degradation rate. For this reason, the preparation and characterization of new liposomes that are able to encapsulate this antibiotic seem to be an important research line in the pharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic structures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium. Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with phosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the encapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes with phosphatidylcholine was measured. The inhibitory zone in an agar plate for free cefepime was similar to that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in working conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All obtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can be used in bacterial infections against Escherichia coli with high inhibitory activity.
SN 1999-4923
YR 2019
FD 2019-02-06
LK http://hdl.handle.net/10668/13537
UL http://hdl.handle.net/10668/13537
LA en
DS RISalud
RD Apr 18, 2025