RT Journal Article
T1 Evolution of Experimental Models in the Study of Glioblastoma: Toward Finding Efficient Treatments
A1 Gómez-Oliva, Ricardo
A1 Domínguez-García, Samuel
A1 Carrascal, Livia
A1 Abalos-Martínez, Jessica
A1 Pardillo-Díaz, Ricardo
A1 Verástegui, Cristina
A1 Castro, Carmen
A1 Nunez-Abades, Pedro
A1 Geribaldi-Doldán, Noelia
K1 Glioma stem cells
K1 Cell cultures of glioma cells
K1 Mouse models of glioblastoma
K1 Brain organoids
K1 3D bioprinting
K1 Brain tumor
K1 Stem Cells
K1 Bioimpresión
K1 Neoplasias encefálicas
K1 Células madre
K1 Extracellular matrix
K1 Matriz extracelular
K1 Glioma
AB Glioblastoma (GBM) is the most common form of brain tumor characterized by its resistance to conventional therapies, including temozolomide, the most widely used chemotherapeutic agent in the treatment of GBM. Within the tumor, the presence of glioma stem cells (GSC) seems to be the reason for drug resistance. The discovery of GSC has boosted the search for new experimental models to study GBM, which allow the development of new GBM treatments targeting these cells. In here, we describe different strategies currently in use to study GBM. Initial GBM investigations were focused in the development of xenograft assays. Thereafter, techniques advanced to dissociate tumor cells into single-cell suspensions, which generate aggregates referred to as neurospheres, thus facilitating their selective expansion. Concomitantly, the finding of genes involved in the initiation and progression of GBM tumors, led to the generation of mice models for the GBM. The latest advances have been the use of GBM organoids or 3D-bioprinted mini-brains. 3D bio-printing mimics tissue cytoarchitecture by combining different types of cells interacting with each other and with extracellular matrix components. These in vivo models faithfully replicate human diseases in which the effect of new drugs can easily be tested. Based on recent data from human glioblastoma, this review critically evaluates the different experimental models used in the study of GB, including cell cultures, mouse models, brain organoids, and 3D bioprinting focusing in the advantages and disadvantages of each approach to understand the mechanisms involved in the progression and treatment response of this devastating disease.
PB Frontiers
YR 2021
FD 2021-01-29
LK http://hdl.handle.net/10668/4110
UL http://hdl.handle.net/10668/4110
LA en
NO Gómez-Oliva R, Domínguez-García S, Carrascal L, Abalos-Martínez J, Pardillo-Díaz R, Verástegui C, et al. Evolution of Experimental Models in the Study of Glioblastoma: Toward Finding Efficient Treatments. Front Oncol. 2021 Jan 29;10:614295
DS RISalud
RD Apr 10, 2025