RT Journal Article
T1 miR-122 direct detection in human serum by time-gated fluorescence imaging.
A1 Garcia-Fernandez, Emilio
A1 Gonzalez-Garcia, M Carmen
A1 Pernagallo, Salvatore
A1 Ruedas-Rama, Maria J
A1 Fara, Mario A
A1 López-Delgado, Francisco J
A1 Dear, James W
A1 Ilyine, Hugh
A1 Ress, Cristina
A1 Díaz-Mochón, Juan J
A1 Orte, Angel
AB A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.
YR 2019
FD 2019-11-27
LK http://hdl.handle.net/10668/14751
UL http://hdl.handle.net/10668/14751
LA en
DS RISalud
RD Apr 11, 2025