RT Journal Article
T1 Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment.
A1 Olivares-Urbano, María Auxiliadora
A1 Griñán-Lisón, Carmen
A1 Ríos-Arrabal, Sandra
A1 Artacho-Cordón, Francisco
A1 Torralbo, Ana Isabel
A1 López-Ruiz, Elena
A1 Marchal, Juan Antonio
A1 Núñez, María Isabel
K1 3D culture
K1 Matrigel
K1 breast cancer outcomes
K1 cancer stem cells
K1 inhibitors
K1 irradiation
K1 matrix metalloproteases
K1 radiotherapy
K1 stemness phenotype
K1 targeted treatment
AB Breast cancer is the most common cancer in women. Radiotherapy (RT) is one of the mainstay treatments for cancer but in some cases is not effective. Cancer stem cells (CSCs) within the tumor can be responsible for recurrence and metastasis after RT. Matrix metalloproteases (MMPs), regulated mainly by tissue inhibitors of metalloproteinases (TIMPs) and histone deacetylases (HDACs), may also contribute to tumor development by modifying its activity after RT. The aim of this work was to study the effects of RT on the expression of MMPs, TIMPs and HDACs on different cell subpopulations in MCF-7, MDA-MB-231 and SK-BR-3 cell lines. We assessed the in vitro expression of these genes in different 3D culture models and induced tumors in female NSG mice by orthotopic xenotransplants. Our results showed that gene expression is related to the cell subpopulation studied, the culture model used and the single radiation dose administered. Moreover, the crucial role played by the microenvironment in terms of cell interactions and CSC plasticity in tumor growth and RT outcome is also shown, supporting the use of higher doses (6 Gy) to achieve better control of tumor development.
SN 2072-6694
YR 2019
FD 2019-11-12
LK https://hdl.handle.net/10668/28123
UL https://hdl.handle.net/10668/28123
LA en
DS RISalud
RD Apr 6, 2025