RT Journal Article
T1 Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats
A1 Casuso, Rafael A.
A1 Al Fazazi, Saad
A1 Plaza-Díaz, Julio
A1 Ruiz-Ojeda, Francisco J.
A1 Rueda-Robles, Ascensión
A1 Aragón-Vela, Jerónimo
A1 Huertas, Jesús R.
K1 Polyphenols
K1 Mitochondria
K1 Glycolysis
K1 Gene expression
K1 Skeletal muscle
K1 Fatty acids
K1 Glucose
K1 mRNA
K1 Olive oil
K1 Polifenoles
K1 Mitocondrias
K1 Glucólisis
K1 Expresión génica
K1 Músculo esquelético
K1 Ácidos grasos
K1 Glucosa
K1 ARN mensajero
K1 Aceite de oliva
AB We tested whether physiological doses of hydroxytyrosol (HT) may alter the mRNA transcription of key metabolic genes in exercised skeletal muscle. Two groups of exercise-trained Wistar rats, HTlow and HTmid, were supplemented with 0.31 and 4.61 mg/kg/d of HT, respectively, for 10 weeks. Another two groups of rats were not supplemented with HT; one remained sedentary and the other one was exercised. After the experimental period, the soleus muscle was removed for qRT-PCR and western blot analysis. The consumption of 4.61 mg/kg/d of HT during exercise increased the mRNA expression of important metabolic proteins. Specifically, 4.61 mg/kg/d of HT may upregulate long-chain fatty acid oxidation, lactate, and glucose oxidation as well as mitochondrial Krebs cycle in trained skeletal muscle. However, a 4.61 mg/kg/d of HT may alter protein translation, as in spite of the increment showed by CD36 and GLUT4 at the mRNA level this was not translated to higher protein content.
PB MDPI
YR 2021
FD 2021-12-12
LK http://hdl.handle.net/10668/4501
UL http://hdl.handle.net/10668/4501
LA en
NO Casuso RA, Al Fazazi S, Plaza-Díaz J, Ruiz-Ojeda FJ, Rueda-Robles A, Aragón-Vela J, et al. Physiological Doses of Hydroxytyrosol Modulate Gene Expression in Skeletal Muscle of Exercised Rats. Life. 2021 Dec 12;11(12):1393
DS RISalud
RD Apr 6, 2025