RT Journal Article
T1 The MRC1/CD68 Ratio Is Positively Associated with Adipose Tissue Lipogenesis and with Muscle Mitochondrial Gene Expression in Humans.
A1 Moreno-Navarrete, José María
A1 Ortega, Francisco
A1 Gómez-Serrano, María
A1 García-Santos, Eva
A1 Ricart, Wifredo
A1 Tinahones, Francisco
A1 Mingrone, Geltrude
A1 Peral, Belén
A1 Fernández-Real, José Manuel
K1 Adipocitos
K1 Expresión Génica
K1 Lipogénesis
K1 Mitocondrias Musculares
K1 Tejido Adiposo
K1 Obesidad
K1 Marcadores Biológicos
K1 Humanos
AB BACKGROUNDAlternative macrophages (M2) express the cluster differentiation (CD) 206 (MCR1) at high levels. Decreased M2 in adipose tissue is known to be associated with obesity and inflammation-related metabolic disturbances. Here we aimed to investigate MCR1 relative to CD68 (total macrophages) gene expression in association with adipogenic and mitochondrial genes, which were measured in human visceral [VWAT, n = 147] and subcutaneous adipose tissue [SWAT, n = 76] and in rectus abdominis muscle (n = 23). The effects of surgery-induced weight loss were also longitudinally evaluated (n = 6).RESULTSMCR1 and CD68 gene expression levels were similar in VWAT and SWAT. A higher proportion of CD206 relative to total CD68 was present in subjects with less body fat and lower fasting glucose concentrations. The ratio MCR1/CD68was positively associated with IRS1gene expression and with the expression of lipogenic genes such as ACACA, FASN and THRSP, even after adjusting for BMI. The ratio MCR1/CD68 in SWAT increased significantly after the surgery-induced weight loss (+44.7%; p = 0.005) in parallel to the expression of adipogenic genes. In addition, SWAT MCR1/CD68ratio was significantly associated with muscle mitochondrial gene expression (PPARGC1A, TFAM and MT-CO3). AT CD206 was confirmed by immunohistochemistry to be specific of macrophages, especially abundant in crown-like structures.CONCLUSIONA decreased ratio MCR1/CD68 is linked to adipose tissue and muscle mitochondrial dysfunction at least at the level of expression of adipogenic and mitochondrial genes.
PB Public Library of Science
YR 2013
FD 2013-08-12
LK http://hdl.handle.net/10668/1274
UL http://hdl.handle.net/10668/1274
LA en
NO Moreno-Navarrete JM, Ortega F, Gómez-Serrano M, García-Santos E, Ricart W, Tinahones F, et al. The MRC1/CD68 Ratio Is Positively Associated with Adipose Tissue Lipogenesis and with Muscle Mitochondrial Gene Expression in Humans. PLoS ONE. 2013; 8(8):e70810
NO Journal Article;
DS RISalud
RD Apr 17, 2025