RT Journal Article
T1 Antimicrobial Activity of the Circular Bacteriocin AS-48 against Clinical Multidrug-Resistant Staphylococcus aureus
A1 Velázquez-Suárez, Cristina
A1 Cebrián, Rubén
A1 Gasca-Capote, Carmen
A1 Sorlózano-Puerto, Antonio
A1 Gutiérrez-Fernández, José
A1 Martínez-Bueno, Manuel
A1 Maqueda, Mercedes
A1 Valdivia, Eva
K1 Staphylococcus aureus MRSA
K1 Enterocin AS-48
K1 Antibiotic resistance
K1 Biofilms
K1 Aminoglycosides
K1 Macrolides
K1 Methicillin
K1 Infections
K1 Bacteriocins
K1 Electron microscopy
K1 Genotype
K1 Staphylococcus aureus resistente a meticilina
K1 Farmacorresistencia microbiana
K1 Biopelículas
K1 Aminoglicósidos
K1 Macrólidos
K1 Meticilina
K1 Infecciones
K1 Bacteriocinas
K1 Microscopía electrónica
K1 Genotipo
AB The treatment and hospital-spread-control of methicillin-resistant Staphylococcus aureus (MRSA) is an important challenge since these bacteria are involved in a considerable number of nosocomial infections that are difficult to treat and produce prolonged hospitalization, thus also increasing the risk of death. In fact, MRSA strains are frequently resistant to all β-lactam antibiotics, and co-resistances with other drugs such as macrolides, aminoglycosides, and lincosamides are usually reported, limiting the therapeutical options. To this must be added that the ability of these bacteria to form biofilms on hospital surfaces and devices confer high antibiotic resistance and favors horizontal gene transfer of genetic-resistant mobile elements, the spreading of infections, and relapses. Here, we genotypically and phenotypically characterized 100 clinically isolated S. aureus for their resistance to 18 antibiotics (33% of them were OXA resistant MRSA) and ability to form biofilms. From them, we selected 48 strains on the basis on genotype group, antimicrobial-resistance profile, and existing OXA resistance to be assayed against bacteriocin AS-48. The results showed that AS-48 was active against all strains, regardless of their clinical source, genotype, antimicrobial resistance profile, or biofilm formation capacity, and this activity was enhanced in the presence of the antimicrobial peptide lysozyme. Finally, we explored the effect of AS-48 on formed S. aureus biofilms, observing a reduction in S. aureus S-33 viability. Changes in the matrix structure of the biofilms as well as in the cell division process were observed with scanning electron microscopy in both S-33 and S-48 S. aureus strains.
PB MDPI
YR 2021
FD 2021-07-30
LK http://hdl.handle.net/10668/4557
UL http://hdl.handle.net/10668/4557
LA en
NO Velázquez-Suárez C, Cebrián R, Gasca-Capote C, Sorlózano-Puerto A, Gutiérrez-Fernández J, Martínez-Bueno M, et al. Antimicrobial Activity of the Circular Bacteriocin AS-48 against Clinical Multidrug-Resistant Staphylococcus aureus. Antibiotics. 2021 Jul 30;10(8):925
DS RISalud
RD Apr 17, 2025