RT Journal Article
T1 In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications. Running Title: Fibrin-Agarose Functionalized Scaffolds.
A1 Blanco-Elices, Cristina
A1 España-Guerrero, Enrique
A1 Mateu-Sanz, Miguel
A1 Sánchez-Porras, David
A1 García-García, Óscar Darío
A1 Sánchez-Quevedo, María Del Carmen
A1 Fernández-Valadés, Ricardo
A1 Alaminos, Miguel
A1 Martín-Piedra, Miguel Ángel
A1 Garzón, Ingrid
K1 biomaterials
K1 extracellular matrix
K1 functionalization
K1 oral and dental tissues
K1 tissue engineering
AB Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin-agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development.
SN 1996-1944
YR 2020
FD 2020-04-04
LK https://hdl.handle.net/10668/26196
UL https://hdl.handle.net/10668/26196
LA en
DS RISalud
RD Apr 8, 2025