RT Journal Article
T1 Systematic evaluation of plasma signaling cascades by functional proteomics approaches: SARS-CoV-2 infection as model.
A1 Juanes-Velasco, Pablo
A1 García-Vaquero, Marina L
A1 Landeira-Viñuela, Alicia
A1 Lopez-Campos, José Luis
A1 Marín, Carmen
A1 Lecrevisse, Quentin
A1 Arias-Hidalgo, Carlota
A1 Montalvillo, Enrique
A1 Góngora, Rafael
A1 Hernández, Ángela-Patricia
A1 Fuentes, Manuel
K1 COVID-19
K1 Functional proteomics
K1 acute phase reactant
K1 antibody
K1 high-throughput screening
K1 immune system
K1 inflammation
K1 microarray
AB Acute phase reactants (APRs) play a critical role in inflammation. The difference in their physiological functions or the different dynamic ranges of these proteins in plasma makes it difficult to detect them simultaneously and to use several of these proteins as a tool in clinical practice. A novel multiplex assay has been designed and optimized to carry out a high-throughput and simultaneous screening of APRs, allowing the detection of each of them at the same time and in their corresponding dynamic range. Using Sars-CoV-2 infection as a model, it has been possible to profile different patterns of acute phase proteins that vary significantly between healthy and infected patients. In addition, severity profiles (acute respiratory distress syndrome and sepsis) have been established. Differential profiles in acute phase proteins can serve as a diagnostic and prognostic tool, among patient stratification. The design of this new platform for their simultaneous detection paves the way for them to be more extensive use in clinical practice.
YR 2022
FD 2022-10-13
LK http://hdl.handle.net/10668/22506
UL http://hdl.handle.net/10668/22506
LA en
DS RISalud
RD Apr 12, 2025