RT Journal Article
T1 Synergistic Effect between Amoxicillin and TLR Ligands on Dendritic Cells from Amoxicillin-Delayed Allergic Patients.
A1 Sanchez-Quintero, Maria J
A1 Torres, Maria J
A1 Blazquez, Ana B
A1 Gómez, Enrique
A1 Fernandez, Tahia D
A1 Doña, Inmaculada
A1 Ariza, Adriana
A1 Andreu, Inmaculada
A1 Melendez, Lidia
A1 Blanca, Miguel
A1 Mayorga, Cristobalina
K1 Amoxicilina
K1 Células dendríticas
K1 Ligandos
K1 Sinergismo medicamentoso
K1 Hipersensibilidad retardada
K1 Linfocitos
K1 Citocinas
K1 Exantema
K1 Humanos
AB Amoxicillin, a low-molecular-weight compound, is able to interact with dendritic cells inducing semi-maturation in vitro. Specific antigens and TLR ligands can synergistically interact with dendritic cells (DC), leading to complete maturation and more efficient T-cell stimulation. The aim of the study was to evaluate the synergistic effect of amoxicillin and the TLR2, 4 and 7/8 agonists (PAM, LPS and R848, respectively) in TLR expression, DC maturation and specific T-cell response in patients with delayed-type hypersensitivity (DTH) reactions to amoxicillin. Monocyte-derived DC from 15 patients with DTH to amoxicillin and 15 controls were cultured with amoxicillin in the presence or absence of TLR2, 4 and 7/8 agonists (PAM, LPS and R848, respectively). We studied TLR1-9 gene expression by RT-qPCR, and DC maturation, lymphocyte proliferation and cytokine production by flow cytometry. DC from both patients and controls expressed all TLRs except TLR9. The amoxicillin plus TLR2/4 or TLR7/8 ligands showed significant differences, mainly in patients: AX+PAM+LPS induced a decrease in TLR2 and AX+R848 in TLR2, 4, 7 and 8 mRNA levels. AX+PAM+LPS significantly increased the percentage of maturation in patients (75%) vs. controls (40%) (p=0.036) and T-cell proliferation (80.7% vs. 27.3% of cases; p=0.001). Moreover, the combinations AX+PAM+LPS and AX+R848 produced a significant increase in IL-12p70 during both DC maturation and T-cell proliferation. These results indicate that in amoxicillin-induced maculopapular exanthema, the presence of different TLR agonists could be critical for the induction of the innate and adaptive immune responses and this should be taken into account when evaluating allergic reactions to these drugs.
PB Public Library of Science
YR 2013
FD 2013-09-16
LK http://hdl.handle.net/10668/1377
UL http://hdl.handle.net/10668/1377
LA en
NO Sanchez-Quintero MJ, Torres MJ, Blazquez AB, Gómez E, Fernandez TD, Doña I, et al. Synergistic Effect between Amoxicillin and TLR Ligands on Dendritic Cells from Amoxicillin-Delayed Allergic Patients. PLoS ONE. 2013; 8(9):e74198
NO Journal Article;
DS RISalud
RD Apr 12, 2025