RT Journal Article
T1 Using NS5B Sequencing for Hepatitis C Virus Genotyping Reveals Discordances with Commercial Platforms.
A1 Chueca, Natalia
A1 Rivadulla, Isidro
A1 Lovatti, Rubén
A1 Reina, Gabriel
A1 Blanco, Ana
A1 Fernandez-Caballero, Jose Angel
A1 Cardeñoso, Laura
A1 Rodriguez-Granjer, Javier
A1 Fernandez-Alonso, Miriam
A1 Aguilera, Antonio
A1 Alvarez, Marta
A1 Galán, Juan Carlos
A1 García, Federico
K1 Antivirales
K1 Genotipo
K1 Técnicas de genotipaje
K1 Hepacivirus
K1 España
AB We aimed to evaluate the correct assignment of HCV genotypes by three commercial methods-Trugene HCV genotyping kit (Siemens), VERSANT HCV Genotype 2.0 assay (Siemens), and Real-Time HCV genotype II (Abbott)-compared to NS5B sequencing. We studied 327 clinical samples that carried representative HCV genotypes of the most frequent geno/subtypes in Spain. After commercial genotyping, the sequencing of a 367 bp fragment in the NS5B gene was used to assign genotypes. Major discrepancies were defined, e.g. differences in the assigned genotype by one of the three methods and NS5B sequencing, including misclassification of subtypes 1a and 1b. Minor discrepancies were considered when differences at subtype levels, other than 1a and 1b, were observed. The overall discordance with the reference method was 34% for Trugene and 15% for VERSANT HCV2.0. The Abbott assay correctly identified all 1a and 1b subtypes, but did not subtype all the 2, 3, 4 and 5 (34%) genotypes. Major discordances were found in 16% of cases for Trugene HCV, and the majority were 1b- to 1a-related discordances; major discordances were found for VERSANT HCV 2.0 in 6% of cases, which were all but one 1b to 1a cases. These results indicated that the Trugene assay especially, and to a lesser extent, Versant HCV 2.0, can fail to differentiate HCV subtypes 1a and 1b, and lead to critical errors in clinical practice for correctly using directly acting antiviral agents.
PB Public Library of Science
YR 2016
FD 2016-04-20
LK http://hdl.handle.net/10668/2322
UL http://hdl.handle.net/10668/2322
LA en
NO Chueca N, Rivadulla I, Lovatti R, Reina G, Blanco A, Fernandez-Caballero JA, et al. Using NS5B Sequencing for Hepatitis C Virus Genotyping Reveals Discordances with Commercial Platforms. PLoS ONE. 2016; 11(4):e0153754
NO Journal Article;
DS RISalud
RD Apr 12, 2025