RT Journal Article
T1 Nanoscale ligand density modulates gap junction intercellular communication of cell condensates during chondrogenesis.
A1 Casanellas, Ignasi
A1 Lagunas, Anna
A1 Vida, Yolanda
A1 Pérez-Inestrosa, Ezequiel
A1 Rodríguez-Pereira, Cristina
A1 Magalhaes, Joana
A1 Andrades, José A
A1 Becerra, José
A1 Samitier, Josep
K1 arginine–glycine–aspartic acid
K1 cell adhesion
K1 condensation
K1 dendrimer-based nanopatterning
K1 gap junction intercellular communication
K1 mesenchymal stem cells
AB Aim: To unveil the influence of cell-matrix adhesions in the establishment of gap junction intercellular communication (GJIC) during cell condensation in chondrogenesis. Materials & methods: Previously developed nanopatterns of the cell adhesive ligand arginine-glycine-aspartic acid were used as cell culture substrates to control cell adhesion at the nanoscale. In vitro chondrogenesis of mesenchymal stem cells was conducted on the nanopatterns. Cohesion and GJIC were evaluated in cell condensates. Results: Mechanical stability and GJIC are enhanced by a nanopattern configuration in which 90% of the surface area presents adhesion sites separated less than 70 nm, thus providing an onset for cell signaling. Conclusion: Cell-matrix adhesions regulate GJIC of mesenchymal cell condensates during in vitro chondrogenesis from a threshold configuration at the nanoscale.
YR 2022
FD 2022-06-01
LK http://hdl.handle.net/10668/20498
UL http://hdl.handle.net/10668/20498
LA en
DS RISalud
RD Apr 8, 2025