RT Journal Article
T1 Impact of TGF-β family-related growth factors on chondrogenic differentiation of adipose-derived stem cells isolated from lipoaspirates and infrapatellar fat pads of osteoarthritic patients.
A1 Lopez-Ruiz, E
A1 Jimenez, G
A1 Kwiatkowski, W
A1 Montañez, E
A1 Arrebola, F
A1 Carrillo, E
A1 Choe, S
A1 Marchal, J A
A1 Peran, M
K1 Adipose Tissue
K1 Adipose stem cells
K1 chondrogenic differentiation
K1 osteoarthritis
K1 transforming growth factor-β family-related growth factors
AB The success of cell-based approaches for the treatment of cartilage defects requires an optimal autologous cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation. The objective of this study was to compare the chondrogenic capacity of mesenchymal stem cells (MSCs) isolated from lipoaspirates (ASCs) and the infrapatellar fat pad (IFPSCs) of osteoarthritic patients and treated with transforming growth factor (TGF)-β family-related growth factors. Cells were cultured for 6 weeks in a 3D pellet culture system with the chimeric activin A/bone morphogenic protein (BMP)-2 ligand (AB235), the chimeric nodal/BMP-2 ligand (NB260) or BMP-2. To investigate the stability of the new cartilage, ASCs-treated pellets were transplanted subcutaneously into severe combined immunodeficiency (SCID) mice. Histological and immunohistochemical assessment confirmed that the growth factors induced cartilage differentiation in both isolated cell types. However, reverse transcription-quantitative PCR results showed that ASCs presented a higher chondrogenic potential than IFPSCs. In vivo results revealed that AB235-treated ASCs pellets were larger in size and could form stable cartilage-like tissue as compared to NB260-treated pellets, while BMP-2-treated pellets underwent calcification. The chondrogenic induction of ASCs by AB235 treatment was mediated by SMAD2/3 activation, as proved by immunofluorescence analysis. The results of this study indicated that the combination of ASCs and AB235 might lead to a cell-based cartilage regeneration treatment.
PB AO Research Institute Davos
YR 2018
FD 2018-04-13
LK http://hdl.handle.net/10668/12342
UL http://hdl.handle.net/10668/12342
LA en
NO The authors gratefully thank Ana Santos, Mohamed Tassi and Gustavo Ortiz from the C.I.C. (University ofGranada) for excellent technical assistance and Shivan Barungi for her help in English language editing. G.J.acknowledges the Junta de Andalucía for providing a post-doctoral fellowship. E.L-R. acknowledges the MINECOfor providing a post-doctoral fellowship through the project RTC-2016-5451. This work was supported by theMinisterio de Economía, Industria y Competitividad (FEDER funds, project RTC-2016-5451-1) and by theConsejería de Economía, Innovación y Ciencia and Fundación Progreso y Salud (Junta de Andalucía, projectnumber PIN-0379-2016).
DS RISalud
RD Apr 8, 2025