RT Journal Article
T1 Pegylated Interferon-α-Induced Natural Killer Cell Activation Is Associated With Human Immunodeficiency Virus-1 DNA Decline in Antiretroviral Therapy-Treated HIV-1/Hepatitis C Virus-Coinfected Patients.
A1 Hua, Stephane
A1 Vigano, Selena
A1 Tse, Samantha
A1 Zhengyu, Ouyang
A1 Harrington, Sean
A1 Negron, Jordi
A1 Garcia-Broncano, Pilar
A1 Marchetti, Giulia
A1 Genebat, Miguel
A1 Leal, Manuel
A1 Resino, Salvador
A1 Ruiz-Mateos, Ezequiel
A1 Lichterfeld, Mathias
A1 Yu, Xu G
K1 Antiretroviral Therapy, Highly Active
K1 Coinfection
K1 HIV Infections
K1 Hepacivirus
K1 Lymphocyte Activation
K1 Polyethylene Glycols
AB BackgroundInterferon alpha (IFN-α) can potently reduce human immunodeficiency virus type 1 ( HIV-1 ) replication in tissue culture and animal models , but may also modulate residual viral reservoirs that persist despite suppressive antiretroviral combination therapy . However, mechanisms leading to viral reservoir reduction during IFN-α treatment are unclear.MethodsWe analyzed HIV-1 gag DNA levels in CD4 T cells by digital droplet polymerase chain reaction and CD8 T-cell and natural killer (NK) cell phenotypes by flow cytometry in a cohort of antiretroviral therapy –treated HIV-1 / hepatitis C virus –coinfected patients (n = 67) undergoing treatment for hepatitis C infection with pegylated IFN-α and ribavirin for an average of 11 months.ResultsWe observed that IFN-α treatment induced a significant decrease in CD4 T-cell counts (P < .0001), in CD4 T-cell –associated HIV-1 DNA copies (P = .002) and in HIV-1 DNA copies per microliter of blood (P < .0001) in our study patients . Notably, HIV-1 DNA levels were unrelated to HIV-1 –specific CD8 T-cell responses. In contrast, proportions of total NK cells , CD56brightCD16–  NK cells , and CD56brightCD16+  NK cells were significantly correlated with reduced levels of CD4 T-cell –associated HIV-1 DNA during IFN-α treatment , especially when coexpressing the activation markers NKG2D and NKp30.ConclusionsThese data suggest that the reduction of viral reservoir cells during treatment with IFN-α is primarily attributable to antiviral activities of NK cells .
PB Oxford University Press
YR 2018
FD 2018-06-15
LK http://hdl.handle.net/10668/11943
UL http://hdl.handle.net/10668/11943
LA en
NO Hua S, Vigano S, Tse S, Zhengyu O, Harrington S, Negron J, et al. Pegylated Interferon-α-Induced Natural Killer Cell Activation Is Associated With Human Immunodeficiency Virus-1 DNA Decline in Antiretroviral Therapy-Treated HIV-1/Hepatitis C Virus-Coinfected Patients. Clin Infect Dis. 2018 Jun 1;66(12):1910-1917.
DS RISalud
RD Aug 21, 2025