RT Journal Article
T1 Preliminary evaluation of a new kit for differentiation of Mycobacterium tuberculosis complex species using Speed-Oligo MTBC.
A1 Lara-Oya, Ana
A1 Rodríguez-Granger, Javier Moisés
A1 Liébana-Martos, María Del Carmen
A1 Mendoza-López, Pablo
A1 Sampedro-Martínez, Antonio
A1 Cobo, Fernando
A1 Gutierrez-Fernández, José
A1 Martínez-Lirola, Miguel José
A1 Navarro-Marí, José María
AB We present the first evaluation of a novel molecular assay, the Speed-Oligo Mycobacterium tuberculosis complex (SO-MTBC), which is based on PCR combined with a dipstick for the differentiation of M. tuberculosis complex (MTBC) members. The results of this assay were compared with findings obtained using the Genotype MTBC assay. In this study, 189 strains of MTBC isolates from 2011 to 2014 were evaluated to determine the MTBC species. Most (174, 92 %) of the strains were identified as M. tuberculosissensu stricto, 7 (3.7 %) as Mycobacteriumbovis, 5 (2.6 %) as M. bovis bacillus Calmette-Guérin, 2 (1.1 %) as Mycobacteriumafricanum and 1 (0.5 %) as Mycobacteriumcaprae; no strains belonged to Mycobacteriummicroti and Mycobacteriumcanettii subsp. The concordance κ coefficient obtained was 0.96 with the results of the Genotype MTBC assay. SO-MTBC may represent a fast and easy-to-use alternative for differentiating among MTBC subspecies in laboratories with standard equipment.
YR 2016
FD 2016-07-06
LK http://hdl.handle.net/10668/10250
UL http://hdl.handle.net/10668/10250
LA en
DS RISalud
RD Apr 17, 2025