RT Journal Article
T1 Fibrinogen-thrombin collagen patch reinforcement of high-risk colonic anastomoses in rats.
A1 Suárez-Grau, Juan Manuel
A1 Bernardos García, Carlos
A1 Cepeda Franco, Carmen
A1 Mendez García, Cristina
A1 García Ruiz, Salud
A1 Docobo Durantez, Fernando
A1 Morales-Conde, Salvador
A1 Padillo Ruiz, Javier
K1 Anastomosis
K1 Colon
K1 Leak
K1 Rats
K1 Surgery
K1 TachoSil®
AB To evaluate the effectiveness of human fibrinogen-thrombin collagen patch (TachoSil®) in the reinforcement of high-risk colon anastomoses. A quasi-experimental study was conducted in Wistar rats (n = 56) that all underwent high-risk anastomoses (anastomosis with only two sutures) after colectomies. The rats were divided into two randomized groups: Control group (24 rats) and treatment group (24 rats). In the treatment group, high-risk anastomosis was reinforced with TachoSil® (a piece of TachoSil® was applied over this high-risk anastomosis, covering the gap). Leak incidence, overall survival, intra-abdominal adhesions, and histologic healing of anastomoses were analyzed. Survivors were divided into two subgroups and euthanized at 15 and 30 d after intervention in order to analyze the adhesions and histologic changes. Overall survival was 71.4% and 57.14% in the TachoSil® group and control group, respectively (P = 0.29); four rats died from other causes and six rats in the treatment group and 10 in the control group experienced colonic leakage (P > 0.05). The intra-abdominal adhesion score was similar in both groups, with no differences between subgroups. We found non-significant differences in the healing process according to the histologic score used in both groups (P = 0.066). In our study, the use of TachoSil® was associated with a non-statistically significant reduction in the rate of leakage in high-risk anastomoses. TachoSil® has been shown to be a safe product because it does not affect the histologic healing process or increase intra-abdominal adhesions.
SN 1948-9366
YR 2016
FD 2016
LK https://hdl.handle.net/10668/26785
UL https://hdl.handle.net/10668/26785
LA en
DS RISalud
RD Apr 12, 2025