RT Journal Article
T1 Workflow of Lotmaria passim isolation: Experimental infection with a low-passage strain causes higher honeybee mortality rates than the PRA-403 reference strain.
A1 Buendía-Abad, María
A1 Higes, Mariano
A1 Martín-Hernández, Raquel
A1 Barrios, Laura
A1 Meana, Aranzazu
A1 Fernández Fernández, Alberto
A1 Osuna, Antonio
A1 De Pablos, Luis Miguel
K1 Experimental infection
K1 Honeybee
K1 Isolation
K1 Lotmaria passim
K1 Low-passage
K1 Trypanosomatid
AB The impact of trypanosomatid parasites on honeybee health may represent a major threat to bee colonies worldwide. However, few axenic isolates have been generated to date and with no details on cell culture passages, a parameter that could influence parasite virulence. To address this question, a trypanosomatid isolation protocol was developed and a new strain was obtained, named L. passim C1. Using experimental infection of worker honeybees, we compared the virulence and mortality rates of the ATCC PRA-403 reference strain and C1 strain, the latter showing higher virulence from 10 days post-infection onward. This study highlights the impact of cell culture passages on the pathogenicity of L. passim in honeybees, providing new evidence of its negative effects on honeybee health.
SN 2213-2244
YR 2020
FD 2020-12-31
LK https://hdl.handle.net/10668/27116
UL https://hdl.handle.net/10668/27116
LA en
DS RISalud
RD Apr 6, 2025