%0 Journal Article
%A Buendía-Abad, María
%A Higes, Mariano
%A Martín-Hernández, Raquel
%A Barrios, Laura
%A Meana, Aranzazu
%A Fernández Fernández, Alberto
%A Osuna, Antonio
%A De Pablos, Luis Miguel
%T Workflow of Lotmaria passim isolation: Experimental infection with a low-passage strain causes higher honeybee mortality rates than the PRA-403 reference strain.
%D 2020
%@ 2213-2244
%U https://hdl.handle.net/10668/27116
%X The impact of trypanosomatid parasites on honeybee health may represent a major threat to bee colonies worldwide. However, few axenic isolates have been generated to date and with no details on cell culture passages, a parameter that could influence parasite virulence. To address this question, a trypanosomatid isolation protocol was developed and a new strain was obtained, named L. passim C1. Using experimental infection of worker honeybees, we compared the virulence and mortality rates of the ATCC PRA-403 reference strain and C1 strain, the latter showing higher virulence from 10 days post-infection onward. This study highlights the impact of cell culture passages on the pathogenicity of L. passim in honeybees, providing new evidence of its negative effects on honeybee health.
%K Experimental infection
%K Honeybee
%K Isolation
%K Lotmaria passim
%K Low-passage
%K Trypanosomatid
%~