RT Journal Article
T1 Cells Deficient in the Fanconi Anemia Protein FANCD2 are Hypersensitive to the Cytotoxicity and DNA Damage Induced by Coffee and Caffeic Acid
A1 Burgos-Moron, Estefania
A1 Manuel Calderon-Montano, Jose
A1 Luis Orta, Manuel
A1 Guillen-mancina, Emilio
A1 Mateos, Santiago
A1 Lopez-Lazaro, Miguel
K1 coffee
K1 caffeic acid
K1 cancer
K1 DNA damage
K1 carcinogenesis
K1 FANCD2
K1 Fanconi anemia
K1 Hydrogen-peroxide
K1 Chlorogenic acid
K1 Epidemiologic evidence
K1 Oxidative stress
K1 In-vitro
K1 Cancer
K1 Consumption
K1 Oxygen
K1 Expression
K1 Beverages
AB Epidemiological studies have found a positive association between coffee consumption and a lower risk of cardiovascular disorders, some cancers, diabetes, Parkinson and Alzheimer disease. Coffee consumption, however, has also been linked to an increased risk of developing some types of cancer, including bladder cancer in adults and leukemia in children of mothers who drink coffee during pregnancy. Since cancer is driven by the accumulation of DNA alterations, the ability of the coffee constituent caffeic acid to induce DNA damage in cells may play a role in the carcinogenic potential of this beverage. This carcinogenic potential may be exacerbated in cells with DNA repair defects. People with the genetic disease Fanconi Anemia have DNA repair deficiencies and are predisposed to several cancers, particularly acute myeloid leukemia. Defects in the DNA repair protein Fanconi Anemia D2 (FANCD2) also play an important role in the development of a variety of cancers (e.g., bladder cancer) in people without this genetic disease. This communication shows that cells deficient in FANCD2 are hypersensitive to the cytotoxicity (clonogenic assay) and DNA damage (gamma-H2AX and 53BP1 focus assay) induced by caffeic acid and by a commercial lyophilized coffee extract. These data suggest that people with Fanconi Anemia, or healthy people who develop sporadic mutations in FANCD2, may be hypersensitive to the carcinogenic activity of coffee.
PB Mdpi
SN 2072-6651
YR 2016
FD 2016-07-01
LK http://hdl.handle.net/10668/19333
UL http://hdl.handle.net/10668/19333
LA en
DS RISalud
RD Apr 7, 2025