RT Journal Article
T1 Influence of Factors Altering Gastric Microbiota on Bariatric Surgery Metabolic Outcomes
A1 Gutierrez-Repiso, Carolina
A1 Moreno-Indias, Isabel
A1 Martin-Nunez, Gracia M.
A1 Ho-Plagaro, Ailec
A1 Ocana-Wilhelmi, Luis
A1 Fernandez Garcia, Diego
A1 Gonzalo Marin, Montserrat
A1 Moreno-Ruiz, Francisco J.
A1 Garcia-Fuentes, Eduardo
A1 Tinahones, Francisco J.
K1 microbiota
K1 stomach
K1 obesity
K1 Helicobacter pylori
K1 proton pump inhibitors
K1 sleeve gastrectomy
K1 Proton pump inhibitors
K1 Mucosa-associated microbiota
K1 Helicobacter-pylori
K1 Sp nov.
AB Little is known about the influence of gastric microbiota on host metabolism, even though the stomach plays an important role in the production of hormones involved in body weight regulation and glucose homeostasis. Proton pump inhibitors (PPIs) and Helicobacter pylori alter gut microbiota, but their impact on gastric microbiota in patients with obesity and the influence of these factors on the metabolic response to bariatric surgery is not fully understood. Forty-one subjects with morbid obesity who underwent sleeve gastrectomy were included in this study. The H. pylori group was established by the detection of H. pylori using a sequencing-based method (n = 16). Individuals in whom H. pylori was not detected were classified according to PPI treatment. Gastric biopsy specimens were obtained during surgery and were analyzed by a high-throughput-sequencing method. Patients were evaluated at baseline and 3, 6, and 12 months after surgery. beta-Diversity measures were able to cluster patients according to their gastric mucosa-associated microbiota composition. H. pylori and PPI treatment are presented as two important factors for gastric mucosa-associated microbiota. H. pylori reduced diversity, while PPIs altered beta-diversity. Both factors induced changes in the gastric mucosa-associated microbiota composition and its predicted functions. PPI users showed lower percentages of change in the body mass index (BMI) in the short term after surgery, while the H. pylori group showed higher glucose levels and lower percentages of reduction in body weight/BMI 1 year after surgery. PPIs and H. pylori colonization could modify the gastric mucosa-associated microbiota, altering its diversity, composition, and predicted functionality. These factors may have a role in the metabolic evolution of patients undergoing bariatric surgery.IMPORTANCE The gut microbiota has been shown to have an impact on host metabolism. In the stomach, factors like proton pump inhibitor treatment and Helicobacter pylori haven been suggested to alter gut microbiota; however, the influence of these factors on the metabolic response to bariatric surgery has not been fully studied. In this study, we highlight the impact of these factors on the gastric microbiota composition. Moreover, proton pump inhibitor treatment and the presence of Helicobacter pylori could have an influence on bariatric surgery outcomes, mainly on body weight loss and glucose homeostasis. Deciphering the relationship between gastric hormones and gastric microbiota and their contributions to bariatric surgery outcomes paves the way to develop gut manipulation strategies to improve the metabolic success of bariatric surgery.
PB Amer soc microbiology
SN 2165-0497
YR 2021
FD 2021-12-01
LK https://hdl.handle.net/10668/27090
UL https://hdl.handle.net/10668/27090
LA en
DS RISalud
RD Apr 19, 2025