RT Journal Article
T1 Progressive changes in composition of lymphocytes in lung tissues from patients with non-small-cell lung cancer.
A1 Del Mar Valenzuela-Membrives, María
A1 Perea-García, Francisco
A1 Sanchez-Palencia, Abel
A1 Ruiz-Cabello, Francisco
A1 Gómez-Morales, Mercedes
A1 Miranda-León, María Teresa
A1 Galindo-Angel, Inmaculada
A1 Fárez-Vidal, María Esther
K1 flow cytometry
K1 immunohistochemistry
K1 immunological response
K1 lung cancer
K1 lymphocyte subsets
AB Immune cell infiltration is a common feature of many human solid tumors. Innate and adaptative immune systems contribute to tumor immunosurveillance. We investigated whether tumors evade immune surveillance by inducing states of tolerance and/or through the inability of some immune subpopulations to effectively penetrate tumor nests. Immunohistochemistry and flow cytometry analysis were used to study the composition and distribution of immune subpopulations in samples of peripheral blood, tumor tissue (TT), adjacent tumor tissue (ATT), distant non-tumor tissue (DNTT), cancer nests, cancer stroma, and invasive margin in 61 non-small-cell lung cancer (NSCLC) patients. A significantly higher percentage of T and B cells and significantly lower percentage of NK cells were detected in TT than in DNTT. Memory T cells (CD4+CD45RO+, CD8+CD45RO+) and activated T cells (CD8+DR+) were more prevalent in TT. Alongside this immune activation, the percentage of T cells with immunosuppressive activity was higher in TT than in DNTT. B- cells were practically non-existent in tumor nests and were preferentially located in the invasive margin. The dominant NK cell phenotype in peripheral blood and DNTT was the cytotoxic phenotype (CD56+ CD16+), while the presence of these cells was significantly decreased in ATT and further decreased in TT. Finally, the immunologic response differed between adenocarcinoma and squamous cell carcinoma and according to the tumor differentiation grade. These findings on the infiltration of innate and adaptative immune cells into tumors contribute to a more complete picture of the immune reaction in NSCLC.
YR 2016
FD 2016
LK http://hdl.handle.net/10668/10491
UL http://hdl.handle.net/10668/10491
LA en
DS RISalud
RD Apr 19, 2025