RT Journal Article
T1 Immunotherapy reduces allergen-mediated CD66b expression and myeloperoxidase levels on human neutrophils from allergic patients.
A1 Aroca, Rocio
A1 Chamorro, Cristina
A1 Vega, Antonio
A1 Ventura, Inmaculada
A1 Gómez, Elisa
A1 Pérez-Cano, Ramón
A1 Blanca, Miguel
A1 Monteseirín, Javier
K1 CEACAM8 protein, human
K1 Cell Adhesion Molecules
K1 GPI-Linked Proteins
K1 Lipopolysaccharides
K1 Peroxidase
K1 Antígenos CD
K1 Moléculas de Adhesión Celular
K1 Hipersensibilidad
K1 Inmunoglobulina E
K1 Neutrófilos
AB CD66b is a member of the carcinoembryonic antigen family, which mediates the adhesion between neutrophils and to endothelial cells. Allergen-specific immunotherapy is widely used to treat allergic diseases, and the molecular mechanisms underlying this therapy are poorly understood. The present work was undertaken to analyze A) the in vitro effect of allergens and immunotherapy on cell-surface CD66b expression of neutrophils from patients with allergic asthma and rhinitis and B) the in vivo effect of immunotherapy on cell-surface CD66b expression of neutrophils from nasal lavage fluid during the spring season. Myeloperoxidase expression and activity was also analyzed in nasal lavage fluid as a general marker of neutrophil activation.RESULTSCD66b cell-surface expression is upregulated in vitro in response to allergens, and significantly reduced by immunotherapy (p<0.001). Myeloperoxidase activity in nasal lavage fluid was also significantly reduced by immunotherapy, as were the neutrophil cell-surface expression of CD66b and myeloperoxidase (p<0.001). Interestingly, CD66b expression was higher in neutrophils from nasal lavage fluid than those from peripheral blood, and immunotherapy reduced the number of CD66+MPO+ cells in nasal lavage fluid. Thus, immunotherapy positive effects might, at least in part, be mediated by the negative regulation of the CD66b and myeloperoxidase activity in human neutrophils.
PB Public Library of Science
YR 2014
FD 2014-04-16
LK http://hdl.handle.net/10668/1893
UL http://hdl.handle.net/10668/1893
LA en
NO Aroca R, Chamorro C, Vega A, Ventura I, Gómez E, Pérez-Cano R, et al. Immunotherapy reduces allergen-mediated CD66b expression and myeloperoxidase levels on human neutrophils from allergic patients. PLoS ONE. 2014; 9(4):e94558
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 18, 2025