RT Journal Article
T1 Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications.
A1 Gimenez-Lirola, Luis Gabriel
A1 Zhang, Jianqiang
A1 Carrillo-Avila, Jose Antonio
A1 Chen, Qi
A1 Magtoto, Ronaldo
A1 Poonsuk, Korakrit
A1 Baum, David H
A1 Piñeyro, Pablo
A1 Zimmerman, Jeffrey
K1 ELISA
K1 PEDV
K1 antibody response
K1 cross-reactivity
K1 multiplex FMIA
K1 recombinant structural proteins
K1 whole virus
AB The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous virus exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among porcine coronaviruses is a major concern for the development of pathogen-specific assays. In this study, we used serum samples (n = 792) from pigs of precisely known infection status and a multiplex fluorescent microbead-based immunoassay and/or enzyme-linked immunoassay platform to characterize the antibody response to PEDV whole-virus (WV) particles and recombinant polypeptides derived from the four PEDV structural proteins, i.e., spike (S), nucleocapsid (N), membrane (M), and envelope (E). Antibody assay cutoff values were selected to provide 100% diagnostic specificity for each target. The earliest IgG antibody response, mainly directed against S1 polypeptides, was observed at days 7 to 10 postinfection. With the exception of nonreactive protein E, we observed similar antibody ontogenies and patterns of seroconversion for S1, N, M, and WV antigens. Recombinant S1 provided the best diagnostic sensitivity, regardless of the PEDV strain, with no cross-reactivity detected against transmissible gastroenteritis virus (TGEV), porcine respiratory coronavirus (PRCV), or porcine deltacoronavirus (PDCoV) pig antisera. The WV particles showed some cross-reactivity to TGEV Miller and TGEV Purdue antisera, while N protein presented some cross-reactivity to TGEV Miller. The M protein was highly cross-reactive to TGEV and PRCV antisera. Differences in the antibody responses to specific PEDV structural proteins have important implications in the development and performance of antibody assays for the diagnosis of PEDV enteric disease.
PB American Society for Microbiology
YR 2017
FD 2017-02-10
LK http://hdl.handle.net/10668/10867
UL http://hdl.handle.net/10668/10867
LA en
NO Gimenez-Lirola LG, Zhang J, Carrillo-Avila JA, Chen Q, Magtoto R, et al. Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications. J Clin Microbiol. 2017 May;55(5):1426-1436.
NO We thank Melisa Spadaro and Elisa Gibert for their assistance during the animal study. This study was supported by Iowa Pork Producers Association funds distributed through the National Pork Board (Des Moines, Iowa, USA).
DS RISalud
RD Apr 8, 2025