RT Journal Article
T1 First morphogenetic analysis of parasite eggs from Schistosomiasis haematobium infected sub-Saharan migrants in Spain and proposal for a new standardised study methodology.
A1 Reguera-Gomez, Marta
A1 Valero, M Adela
A1 Oliver-Chiva, M Carmen
A1 de Elias-Escribano, Alejandra
A1 Artigas, Patricio
A1 Cabeza-Barrera, M Isabel
A1 Salas-Coronas, Joaquín
A1 Boissier, Jérôme
A1 Mas-Coma, Santiago
A1 Bargues, M Dolores
K1 Eggs
K1 Phenotypic analysis,
K1 S. haematobium S. bovis
K1 S. mansoni
K1 Spain
K1 Standardised methodology,
K1 Sub-Saharan migrants
AB Schistosomiasis is a Neglected Tropical Disease caused by trematode species of the genus Schistosoma. Both, autochthonous and imported cases of urogenital schistosomiasis have been described in Europe. The present study focuses on eggs, considered pure S. haematobium by genetic characterisation (intergenic ITS region of the rDNA and cox1 mtDNA). A phenotypic characterisation of S. haematobium eggs was made by morphometric comparison with experimental populations of S. bovis and S. mansoni, to help in the diagnosis of S. haematobium populations infecting sub-Saharan migrants in Spain. Analyses were made by Computer Image Analysis System (CIAS) applied on the basis of new standardised measurements and geometric morphometric tools. The principal component analysis (PCA), including seventeen non-redundant measurements, showed three phenotypic patterns in eggs of S. haematobium, S. bovis and S. mansoni. PCA showed that the S. bovis population presented a large egg size range with a pronouncedly larger maximum size. Similarly, S. bovis shows bigger spine values than S. haematobium. Mahalanobis distances between each pair of groups were calculated for each discriminant analysis performed. In general, S. mansoni and S. bovis present larger distances between them than with S. haematobium, i.e. they present the greatest differences. Regarding the spine, S. haematobium and S. mansoni are the most distant species. Results show the usefulness of this methodology for the phenotypic differentiation between eggs from these Schistosoma species, capable of discerning morphologically close eggs, as is the case of the haematobium group. Schistosoma egg phenotyping approaches may be applied to assess not only hybrid forms but also potential influences of a variety of other factors.
YR 2021
FD 2021-08-03
LK https://hdl.handle.net/10668/24808
UL https://hdl.handle.net/10668/24808
LA en
DS RISalud
RD Apr 17, 2025