RT Journal Article
T1 Immune cell impact of three differently coated lipid nanocapsules: pluronic, chitosan and polyethylene glycol.
A1 Farace, Cristiano
A1 Sánchez-Moreno, Paola
A1 Orecchioni, Marco
A1 Manetti, Roberto
A1 Sgarrella, Francesco
A1 Asara, Yolande
A1 Peula-García, José M.
A1 Marchal, Juan A.
A1 Madeddu, Roberto
A1 Delogu, Lucia G.
K1 Quitosano
K1 Citocinas
K1 Citometría de flujo
K1 Humanos
K1 Inmunidad innata
K1 Inmunomodulación
K1 Inmunoterapia
K1 Ácido láctico
K1 Leucocitos mononucleares
K1 Lípidos
K1 Microscopía confocal
K1 Monocitos
K1 Nanocápsulas
K1 Poloxámero
K1 Polietilenglicoles
K1 Polímeros
K1 Linfocitos t
AB Lipid nanocapsules (NCs) represent promising tools in clinical practice for diagnosis and therapy applications. However, the NC appropriate functionalization is essential to guarantee high biocompatibility and molecule loading ability. In any medical application, the immune system-impact of differently functionalized NCs still remains to be fully understood. A comprehensive study on the action exerted on human peripheral blood mononuclear cells (PBMCs) and major immune subpopulations by three different NC coatings: pluronic, chitosan and polyethylene glycol-polylactic acid (PEG) is reported. After a deep particle characterization, the uptake was assessed by flow-cytometry and confocal microscopy, focusing then on apoptosis, necrosis and proliferation impact in T cells and monocytes. Cell functionality by cell diameter variations, different activation marker analysis and cytokine assays were performed. We demonstrated that the NCs impact on the immune cell response is strongly correlated to their coating. Pluronic-NCs were able to induce immunomodulation of innate immunity inducing monocyte activations. Immunomodulation was observed in monocytes and T lymphocytes treated with Chitosan-NCs. Conversely, PEG-NCs were completely inert. These findings are of particular value towards a pre-selection of specific NC coatings depending on biomedical purposes for pre-clinical investigations; i.e. the immune-specific action of particular NC coating can be excellent for immunotherapy applications.
PB Nature Publishing Group
YR 2016
FD 2016-01-05
LK http://hdl.handle.net/10668/2189
UL http://hdl.handle.net/10668/2189
LA en
NO Farace C, Sánchez-Moreno P, Orecchioni M, Manetti R, Sgarrella F, Asara Y, et al. Immune cell impact of three differently coated lipid nanocapsules: pluronic, chitosan and polyethylene glycol. Sci Rep. 2016; 6:18423
NO Journal Article;
DS RISalud
RD Apr 6, 2025