RT Journal Article
T1 Rapid and simultaneous determination of histidine metabolism intermediates in human and mouse microbiota and biomatrices.
A1 Acuña, Inmaculada
A1 Ruiz, Alicia
A1 Cerdo, Tomas
A1 Cantarero, Samuel
A1 Lopez-Moreno, Ana
A1 Aguilera, Margarita
A1 Campoy, Cristina
A1 Suarez, Antonio
K1 UHPLC-ESI-MS/MS
K1 feces
K1 histidine pathway
K1 microbiota
K1 urine
AB Histidine metabolism is a key pathway physiologically involved in satiety, recognition memory, skin, and neural protection and allergic diseases. Microbiologically-produced imidazole propionate induces type II diabetes and interferes with glucose lowering drugs. Despite their determinant health implications, no single method simultaneously assesses histidine metabolites in urine, feces, and microbiota. The aim of this study was to develop a simple, rapid, and sensitive method for the determination of histidine and its major bioactive metabolites histamine, N-acetylhistamine, imidazole-4-acetate, cis-urocanate, trans-urocanate, glutamate and imidazole propionate, using ultrahigh-performance liquid chromatography with electrospray ionization tandem mass spectrometry. An innovative simple extraction method from small aliquots of human and mice urine, feces and microbial cell extracts was coupled to separation in a 6.5 min chromatographic run. The successful performance allowed accurate and precise quantification of all metabolites in mouse feces, suggesting broad exchange of histidine metabolites between the gut and mice. Higher urine histamine, histamine to histidine ratio, and imidazole-4-acetate pointed to an underlying inflammatory or allergic process in mice compared to human subjects. N-acetylhistamine and imidazole propionate were detected in human and mouse feces, confirming its origin from gut microbial metabolism. Our novel and robust analytical method captured histidine metabolism in a single assay that will facilitate broad and deep histidine metabolic phenotyping assessing the impact of microbiota on host health in large-scale human observational and interventional studies.
PB Wiley-Blackwell Publishing, Inc.
YR 2021
FD 2021-06-08
LK http://hdl.handle.net/10668/18184
UL http://hdl.handle.net/10668/18184
LA en
NO Acuña I, Ruiz A, Cerdó T, Cantarero S, López-Moreno A, Aguilera M, et al. Rapid and simultaneous determination of histidine metabolism intermediates in human and mouse microbiota and biomatrices. Biofactors. 2022 Mar;48(2):315-328.
NO The authors are grateful to the Spanish Ministry of Education, Culture and Sports for the pre-doctoral fellowship granted to Inmaculada Acuña (FPU16/04587). Inmaculada Acuña participated in the PhD Program in Nutrition and Food Science of the University of Granada. The results of this manuscript are part of Inmaculada Acuña PhD thesis. This work was carried out within the frame of GP/EFSA/ENCO/380 2018/03/G04: OBEMIRISK: Knowledge platform for assessing the risk of Bisphenols on gut microbiota and its role in obesogenic phenotype: looking for biomarkers. This research was also funded by FEDER-Infrastructure-Consejería de Economía, Conocimiento, Empresas y Universidad: IE_2019-198.
DS RISalud
RD Apr 7, 2025