RT Journal Article
T1 Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering.
A1 Blanco-Elices, Cristina
A1 Chato-Astrain, Jesus
A1 Gonzalez-Gonzalez, Alberto
A1 Sanchez-Porras, David
A1 Carriel, Victor
A1 Fernandez-Valades, Ricardo
A1 Sanchez-Quevedo, Maria Del Carmen
A1 Alaminos, Miguel
A1 Garzon, Ingrid
K1 MSC
K1 umbilical cord
K1 vascular differentiation
AB The embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. The aim of this work is to characterize the hUC at in situ and ex vivo levels to stablish their potential use in vascular regeneration. Human umbilical cords were obtained and histologically prepared for in the situ analysis of four hUC regions (intervascular-IV, perivascular-PV, subaminoblastic-SAM, and Wharton's jelly-WH), and primary cell cultures of mesenchymal stem cells (hUC-MSC) isolated from each region were obtained. The results confirmed the heterogeneity of the hUC, with the IV and PV zones tending to show the higher in situ expression of several components of the extracellular matrix (collagens, proteoglycans, and glycosaminoglycans), vimentin, and MSC markers (especially CD73), although isolation and ex vivo culture resulted in a homogeneous cell profile. Three vascular markers were positive in situ, especially vWF, followed by CD34 and CD31, and isolation and culture revealed that the region associated with the highest expression of vascular markers was IV, followed by PV. These results confirm the heterogeneity of the hUC and the need for selecting cells from specific regions of the hUC for particular applications in tissue engineering.
PB MDPI AG
SN 2075-4426
YR 2022
FD 2022-04-15
LK http://hdl.handle.net/10668/21376
UL http://hdl.handle.net/10668/21376
LA en
NO Blanco-Elices C, Chato-Astrain J, González-González A, Sánchez-Porras D, Carriel V, Fernández-Valadés R, et al. Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering. J Pers Med. 2022 Apr 18;12(4):648.
NO This work was supported by the Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i) of the Spanish Ministry of Science and Innovation (Instituto de Salud Carlos III), Grants FIS PI21/0980, FIS PI18/0331, FIS PI18/0332, FIS PI20/0317, FIS PI20/318, and ICI19-00024, co-financed by FEDER funds (European Union). It was also supported by grant PE-0395-2019 from Consejería de Salud y Familias, Junta de Andalucía, Spain, and grants B-CTS-450-UGR20 and A-CTS-498-UGR18 (Proyectos de I+D+i en el marco del Programa Operativo FEDER Andalucía 2014-2020) from the University of Granada, Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía and European Union and P18-RT-5059 from Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía. Co-financed by FEDER funds (European Union).
DS RISalud
RD Apr 8, 2025