RT Journal Article
T1 D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome
A1 Pericuesta, Eva
A1 Laguna-Barraza, Ricardo
A1 Ramos-Ibeas, Priscila
A1 Gutierrez-Arroyo, Julia L.
A1 Navarro, Juan A.
A1 Vera, Katia
A1 Sanjuan, Carlos
A1 Baixeras, Elena
A1 Rodríguez de Fonseca, Fernando
A1 Gutierrez-Adan, Alfonso
K1 Polycystic ovary syndrome
K1 D-chiro-inositol
K1 Oocyte
K1 Embryo
K1 Fertility
K1 Oxidative stress
K1 Glucose intolerance
K1 Síndrome del ovario poliquístico
K1 Oocitos
K1 Estructuras embrionarias
K1 Fertilidad
K1 Estrés oxidativo
K1 Intolerancia a la glucosa
AB Polycystic ovarian syndrome (PCOS) is the main cause of female infertility. It is a multifactorial disorder with varying clinical manifestations including metabolic/endocrine abnormalities, hyperandrogenism, and ovarian cysts, among other conditions. D-Chiro-inositol (DCI) is the main treatment available for PCOS in humans. To address some of the mechanisms of this complex disorder and its treatment, this study examines the effect of DCI on reproduction during the development of different PCOS-associated phenotypes in aged females and two mouse models of PCOS. Aged females (8 months old) were treated or not (control) with DCI for 2 months. PCOS models were generated by treatment with dihydrotestosterone (DHT) on Days 16, 17, and 18 of gestation, or by testosterone propionate (TP) treatment on the first day of life. At two months of age, PCOS mice were treated with DCI for 2 months and their reproductive parameters analyzed. No effects of DCI treatment were produced on body weight or ovary/body weight ratio. However, treatment reduced the number of follicles with an atretic cyst-like appearance and improved embryo development in the PCOS models, and also increased implantation rates in both aged and PCOS mice. DCI modified the expression of genes related to oocyte quality, oxidative stress, and luteal sufficiency in cumulus-oocyte complexes (COCs) obtained from the aged and PCOS models. Further, the phosphorylation of AKT, a main metabolic sensor activated by insulin in the liver, was enhanced only in the DHT group, which was the only PCOS model showing glucose intolerance and AKT dephosphorylation. The effect of DCI in the TP model seemed mediated by its influence on oxidative stress and follicle insufficiency. Our results indicate that DCI works in preclinical models of PCOS and offer insight into its mechanism of action when used to treat this infertility-associated syndrome.
PB MDPI
YR 2020
FD 2020-08-22
LK http://hdl.handle.net/10668/3863
UL http://hdl.handle.net/10668/3863
LA en
NO Pericuesta E, Laguna-Barraza R, Ramos-Ibeas P, Gutierrez-Arroyo JL, Navarro JA, Vera K, et al. D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome. Int J Mol Sci. 2020 Aug 22;21(17):6049
DS RISalud
RD Apr 10, 2025