RT Journal Article
T1 Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii.
A1 Lazaro-Diez, Maria
A1 Chapartegui-Gonzalez, Itziar
A1 Redondo-Salvo, Santiago
A1 Leigh, Chike
A1 Merino, David
A1 Segundo, David San
A1 Fernandez, Adrian
A1 Navas, Jesus
A1 Icardo, Jose Manuel
A1 Acosta, Felix
A1 Ocampo-Sosa, Alain
A1 Martinez-Martinez, Luis
A1 Ramos-Vivas, Jose
K1 Acinetobacter
K1 Acinetobacter Infections
K1 Acinetobacter baumannii
K1 Extracellular traps
AB Acinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogenesis is scarce. Also, there are no detailed published reports on the interactions between A. pittii and human phagocytic cells. Using confocal laser and scanning electron microscopy, immunofluorescence, and live-cell imaging, our study shows that immediately after bacteria-cell contact, neutrophils rapidly and continuously engulf and kill bacteria during at least 4 hours of infection in vitro. After 3 h of infection, neutrophils start to release neutrophil extracellular traps (NETs) against Acinetobacter. DNA in NETs colocalizes well with human histone H3 and with the specific neutrophil elastase. We have observed that human neutrophils use large filopodia as cellular tentacles to sense local environment but also to detect and retain bacteria during phagocytosis. Furthermore, co-cultivation of neutrophils with human differentiated macrophages before infections shows that human neutrophils, but not macrophages, are key immune cells to control Acinetobacter. Although macrophages were largely activated by both bacterial species, they lack the phagocytic activity demonstrated by neutrophils.
PB Nature Publishing Group
YR 2017
FD 2017-05-22
LK http://hdl.handle.net/10668/11374
UL http://hdl.handle.net/10668/11374
LA en
NO Lázaro-Díez M, Chapartegui-González I, Redondo-Salvo S, Leigh C, Merino D, Segundo DS, et al. Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii. Sci Rep. 2017 Jul 4;7(1):4571. doi: 10.1038/s41598-017-04870-8. Erratum in: Sci Rep. 2020 Mar 11;10(1):4797
NO M.L.-D. holds a contract from the Instituto de Investigación Sanitaria Valdecilla IDIVAL and Universidad de Cantabria (PREVAL16/05). S.R.-S. holds a contract from the Instituto de Investigación Valdecilla IDIVAL.J.R.-V. holds a Miguel Servet II contract for Young Researchers from the Instituto de Salud Carlos III, Spain. Te authors thank Dr. Fidel Madrazo (Electron Microscopy Unit, Technology Support Services, IDIVAL) forhelping with confocal microscopy and live cell imaging. J.R.-V. acknowledges the receipt of a Sociedad Española de Enfermedades Infecciosas y Microbiologı́a Clı́nica (SEIMC) fellowship. J.R.-V. thanks Inés Montes andAdrián Fernández for technical assistance. J.R.-V. was supported by the Spanish Instituto de Salud Carlos III, Spain (grants PI13/01310 and PI16/01103). Research in our laboratories is supported by Plan Nacional deI+D+i 2008–2011 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía y Competitividad, Spanish Network for Research in Infectious Diseases(REIPI RD12/0015) - co-fnanced by European Development Regional Fund “A way to achieve Europe” ERDF. Te funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
DS RISalud
RD Apr 7, 2025