RT Journal Article
T1 Identification of Reference Genes for Quantitative Real Time PCR Assays in Aortic Tissue of Syrian Hamsters with Bicuspid Aortic Valve.
A1 Rueda-Martinez, Carmen
A1 Fernandez, M Carmen
A1 Soto-Navarrete, Maria Teresa
A1 Jimenez-Navarro, Manuel
A1 Duran, Ana Carmen
A1 Fernandez, Borja
K1 Hamsters
K1 Aorta
K1 Gene expression
K1 Aortic valve
K1 Polymerase chain reaction
K1 Computer software
K1 Algorithms
K1 Epidemiology
AB Bicuspid aortic valve (BAV) is the most frequent congenital cardiac malformation in humans, and appears frequently associated with dilatation of the ascending aorta. This association is likely the result of a common aetiology. Currently, a Syrian hamster strain with a relatively high (∼40%) incidence of BAV constitutes the only spontaneous animal model of BAV disease. The characterization of molecular alterations in the aorta of hamsters with BAV may serve to identify pathophysiological mechanisms and molecular markers of disease in humans. In this report, we evaluate the expression of ten candidate reference genes in aortic tissue of hamsters in order to identify housekeeping genes for normalization using quantitative real time PCR (RT-qPCR) assays. A total of 51 adult (180-240 days old) and 56 old (300-440 days old) animals were used. They belonged to a control strain of hamsters with normal, tricuspid aortic valve (TAV; n = 30), or to the affected strain of hamsters with TAV (n = 45) or BAV (n = 32). The expression stability of the candidate reference genes was determined by RT-qPCR using three statistical algorithms, GeNorm, NormFinder and Bestkeeper. The expression analyses showed that the most stable reference genes for the three algorithms employed were Cdkn1β, G3pdh and Polr2a. We propose the use of Cdkn1β, or both Cdkn1β and G3pdh as reference genes for mRNA expression analyses in Syrian hamster aorta.
PB Public Library of Science
YR 2016
FD 2016-10-06
LK http://hdl.handle.net/10668/10510
UL http://hdl.handle.net/10668/10510
LA en
NO Rueda-Martínez C, Fernández MC, Soto-Navarrete MT, Jiménez-Navarro M, Durán AC, Fernández B. Identification of Reference Genes for Quantitative Real Time PCR Assays in Aortic Tissue of Syrian Hamsters with Bicuspid Aortic Valve. PLoS One. 2016 Oct 6;11(10):e0164070.
DS RISalud
RD Oct 8, 2025