RT Conference Proceedings
T1 Altered expression of the spliceosome components in leukocytes subsets from patients with ankylosing spondylitis: association to disease pathogenesis and therapeutic response
A1 Ladehesa, L.
A1 Castro, M. G.
A1 Lopez-Pedrera, C.
A1 Ortega, R.
A1 Pedraza, S.
A1 del Rio, M.
A1 Abalos-Aguilera, M. C.
A1 Ruiz-Limon, P.
A1 Perez-Sanchez, C.
A1 Ibanez-Costa, A.
A1 Parra-Manso, Y.
A1 Barbarroja, N.
A1 Arias-de la Rosa, I.
A1 Font, P.
A1 Escudero, A.
A1 Castano, J. P.
A1 Luque, R. M.
A1 Collantes, E.
A1 Jimenez-Gomez, Y.
K1 Spondylitis, Ankylosing
K1 Spliceosomes
K1 Cross-Sectional Studies
K1 Tumor Necrosis Factor Inhibitors
AB Ankylosing spondylitis (AS) is a chronic inflammatory disease whose etiopathogenesis remains incompletely understood. Splicing, a posttranscriptional process essential for RNA maturation, has recently been implicated in several human diseases through pathological deregulation of the spliceosome; however, alterations of the spliceosome and their modulation by therapeutic responses have not yet been described in AS. The objectives of this study were: (1) to evaluate the potential deregulation of the spliceosome in leukocytes from AS patients and its involvement in disease pathophysiology, and (2) to analyze the in vivo effects of anti-TNF drugs on spliceosome components in these cells. A cross-sectional study was conducted with 32 AS patients and 29 healthy donors (HDs), and a three-month longitudinal study was performed in eight AS patients undergoing anti-TNFα therapy. Disease activity was assessed using the BASDAI index, along with CRP and ESR levels; physical function was measured by BASFI, spinal mobility by BASMI, and structural damage by mSASSS. The expression of selected components of the major (n=12) and minor (n=4) spliceosomes, as well as splicing factors (n=28), was evaluated in purified leukocytes using Fluidigm technology, while inflammatory marker expression was assessed in parallel by RT-PCR.
PB BMJ publishing group
SN 0003-4967
YR 2022
FD 2022-01-09
LK https://hdl.handle.net/10668/28325
UL https://hdl.handle.net/10668/28325
LA en
NO Ladehesa L, Castro M, Lopez-Pedrera C, et al FRI0157 Altered expression of the spliceosome components in leukocytes subsets from patients with ankylosing spondylitis: association to disease pathogenesis and therapeutic responseAnnals of the Rheumatic Diseases 2018;77:621-622
DS RISalud
RD Aug 21, 2025