RT Journal Article
T1 Expression profiling of rectal tumors defines response to neoadjuvant treatment related genes.
A1 Palma, Pablo
A1 Cano, Carlos
A1 Conde-Muiño, Raquel
A1 Comino, Ana
A1 Bueno, Pablo
A1 Ferrón, J Antonio
A1 Cuadros, Marta
K1 Quimiorradioterapia
K1 Radiotolerancia
K1 Neoplasias del recto
K1 Período preoperatorio
K1 Resultado del tratamiento
K1 Perfiles de expresión génica
K1 Marcadores biológicos
K1 Tratamiento neoadyuvante
K1 Análisis de secuencias por matrices de oligonucleótidos
AB To date, no effective method exists that predicts the response to preoperative chemoradiation (CRT) in locally advanced rectal cancer (LARC). Nevertheless, identification of patients who have a higher likelihood of responding to preoperative CRT could be crucial in decreasing treatment morbidity and avoiding expensive and time-consuming treatments. The aim of this study was to identify signatures or molecular markers related to response to pre-operative CRT in LARC. We analyzed the gene expression profiles of 26 pre-treatment biopsies of LARC (10 responders and 16 non-responders) without metastasis using Human WG CodeLink microarray platform. Two hundred and fifty seven genes were differentially over-expressed in the responder patient subgroup. Ingenuity Pathway Analysis revealed a significant ratio of differentially expressed genes related to cancer, cellular growth and proliferation pathways, and c-Myc network. We demonstrated that high Gng4, c-Myc, Pola1, and Rrm1 mRNA expression levels was a significant prognostic factor for response to treatment in LARC patients (p<0.05). Using this gene set, we were able to establish a new model for predicting the response to CRT in rectal cancer with a sensitivity of 60% and 100% specificity. Our results reflect the value of gene expression profiling to gain insight about the molecular pathways involved in the response to treatment of LARC patients. These findings could be clinically relevant and support the use of mRNA levels when aiming to identify patients who respond to CRT therapy.
PB Public Library of Science
YR 2014
FD 2014-11-07
LK http://hdl.handle.net/10668/1898
UL http://hdl.handle.net/10668/1898
LA en
NO Palma P, Cano C, Conde-Muiño R, Comino A, Bueno P, Ferrón JA, et al. Expression profiling of rectal tumors defines response to neoadjuvant treatment related genes. PLoS ONE. 2014; 9(11):e112189
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 7, 2025