RT Journal Article
T1 Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13.
A1 Pacios, Olga
A1 Fernandez-Garcia, Laura
A1 Bleriot, Ines
A1 Blasco, Lucia
A1 Ambroa, Anton
A1 Lopez, Maria
A1 Ortiz-Cartagena, Concha
A1 Cuenca, Felipe Fernandez
A1 Oteo-Iglesias, Jesus
A1 Pascual, Alvaro
A1 Martinez-Martinez, Luis
A1 Domingo-Calap, Pilar
A1 Tomas, Maria
K1 Klebsiella pneumoniae
K1 L-shaped tail fiber
K1 Genomic annotation
K1 Homing endonucleases
K1 Lytic phages
AB Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.
PB MDPI
YR 2021
FD 2021-12-17
LK http://hdl.handle.net/10668/21597
UL http://hdl.handle.net/10668/21597
LA en
NO Pacios O, Fernández-García L, Bleriot I, Blasco L, Ambroa A, López M, et al. Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13. Viruses. 2021 Dec 21;14(1):6
DS RISalud
RD Apr 10, 2025