%0 Journal Article
%A Pacios, Olga
%A Fernandez-Garcia, Laura
%A Bleriot, Ines
%A Blasco, Lucia
%A Ambroa, Anton
%A Lopez, Maria
%A Ortiz-Cartagena, Concha
%A Cuenca, Felipe Fernandez
%A Oteo-Iglesias, Jesus
%A Pascual, Alvaro
%A Martinez-Martinez, Luis
%A Domingo-Calap, Pilar
%A Tomas, Maria
%T Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13.
%D 2021
%U http://hdl.handle.net/10668/21597
%X Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.
%K Klebsiella pneumoniae
%K L-shaped tail fiber
%K Genomic annotation
%K Homing endonucleases
%K Lytic phages
%~