RT Journal Article
T1 Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox
A1 Vítor, Alexandra C.
A1 Huertas, Pablo
A1 Legube, Gaëlle
A1 de Almeida, Sérgio F.
K1 DNA repair
K1 Homologous recombination (HR)
K1 Non-homologous DNA end joining
K1 Chromatin
K1 DNA damage
K1 Reparación del ADN
K1 Recombinación homóloga
K1 Reparación del ADN por unión de extremidades
K1 Cromatina
K1 Daño del ADN
K1 Roturas del ADN de doble cadena
AB To ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling networks comprise the DNA damage response (DDR). The current knowledge of the molecular determinants and mechanistic details of the DDR owes greatly to the continuous development of ground-breaking experimental tools that couple the controlled induction of DSBs at distinct genomic positions with assays and reporters to investigate DNA repair pathways, their impact on other DNA-templated processes and the specific contribution of the chromatin environment. In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR.
PB Frontiers
YR 2020
FD 2020-02-21
LK http://hdl.handle.net/10668/3734
UL http://hdl.handle.net/10668/3734
LA en
NO Vítor AC, Huertas P, Legube G, de Almeida SF. Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox. Front Mol Biosci. 2020 Feb 21;7:24
DS RISalud
RD Apr 10, 2025