%0 Journal Article
%A Vítor, Alexandra C.
%A Huertas, Pablo
%A Legube, Gaëlle
%A de Almeida, Sérgio F.
%T Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox
%D 2020
%U http://hdl.handle.net/10668/3734
%X To ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling networks comprise the DNA damage response (DDR). The current knowledge of the molecular determinants and mechanistic details of the DDR owes greatly to the continuous development of ground-breaking experimental tools that couple the controlled induction of DSBs at distinct genomic positions with assays and reporters to investigate DNA repair pathways, their impact on other DNA-templated processes and the specific contribution of the chromatin environment. In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR.
%K DNA repair
%K Homologous recombination (HR)
%K Non-homologous DNA end joining
%K Chromatin
%K DNA damage
%K Reparación del ADN
%K Recombinación homóloga
%K Reparación del ADN por unión de extremidades
%K Cromatina
%K Daño del ADN
%K Roturas del ADN de doble cadena
%~