RT Journal Article
T1 In1-ghrelin splicing variant is overexpressed in pituitary adenomas and increases their aggressive features.
A1 Ibáñez-Costa, Alejandro
A1 Gahete, Manuel D
A1 Rivero-Cortés, Esther
A1 Rincón-Fernández, David
A1 Nelson, Richard
A1 Beltrán, Manuel
A1 de la Riva, Andrés
A1 Japón, Miguel A
A1 Venegas-Moreno, Eva
A1 Gálvez, Ma Ángeles
A1 García-Arnés, Juan A
A1 Soto-Moreno, Alfonso
A1 Morgan, Jennifer
A1 Tsomaia, Natia
A1 Culler, Michael D
A1 Dieguez, Carlos
A1 Castaño, Justo P
A1 Luque, Raúl M
K1 Adenoma
K1 Corticotropina
K1 Empalme alternativo
K1 Animales
K1 Apoptosis
K1 Inmunotransferencia Western
K1 Células CHO
K1 Línea celular tumoral
K1 Cricetinae
K1 Cinasas MAP reguladas por señales extracelulares
K1 Regulación de la expresión génica neoplásica
K1 Ghrelina
K1 Hormona del crecimiento
K1 Humanos
K1 Intrones
K1 Datos de Secuencia Molecular
K1 Péptidos
K1 Neoplasias hipofisarias
K1 Isoformas de proteínas
K1 Proteínas protooncogénicas c-akt
K1 Interferencia por ARN
K1 Reacción en cadena de la polimerasa por transcriptasa inversa
K1 Transducción de señales
K1 Células tumorales cultivadas
AB Pituitary adenomas comprise a heterogeneous subset of pathologies causing serious comorbidities, which would benefit from identification of novel, common molecular/cellular biomarkers and therapeutic targets. The ghrelin system has been linked to development of certain endocrine-related cancers. Systematic analysis of the presence and functional implications of some components of the ghrelin system, including native ghrelin, receptors and the recently discovered splicing variant In1-ghrelin, in human normal pituitaries (n = 11) and pituitary adenomas (n = 169) revealed that expression pattern of ghrelin system suffers a clear alteration in pituitary adenomasas comparedwith normal pituitary, where In1-ghrelin is markedly overexpressed. Interestingly, in cultured pituitary adenoma cells In1-ghrelin treatment (acylated peptides at 100 nM; 24–72 h) increased GH and ACTH secretion, Ca2+ and ERK1/2 signaling and cell viability, whereas In1-ghrelin silencing (using a specific siRNA; 100 nM) reduced cell viability. These results indicate that an alteration of the ghrelin system, specially its In1-ghrelin variant, could contribute to pathogenesis of different pituitary adenomas types, and suggest that this variant and its related ghrelin system could provide new tools to identify novel, more general diagnostic, prognostic and potential therapeutic targets in pituitary tumors.
PB Nature Publishing Group
YR 2015
FD 2015-03-04
LK http://hdl.handle.net/10668/2264
UL http://hdl.handle.net/10668/2264
LA en
NO Ibáñez-Costa A, Gahete MD, Rivero-Cortés E, Rincón-Fernández D, Nelson R, Beltrán M, et al. In1-ghrelin splicing variant is overexpressed in pituitary adenomas and increases their aggressive features. Sci Rep. 2015; 5:8714
DS RISalud
RD Apr 10, 2025