RT Journal Article
T1 Culturing and Molecular Approaches for Identifying Microbiota Taxa Impacting Children's Obesogenic Phenotypes Related to Xenobiotic Dietary Exposure.
A1 Lopez-Moreno, Ana
A1 Ruiz-Moreno, Angel
A1 Pardo-Cacho, Jesus
A1 Cerk, Klara
A1 Torres-Sanchez, Alfonso
A1 Ortiz, Pilar
A1 Ubeda, Marina
A1 Aguilera, Margarita
K1 BPA
K1 bioinformatics
K1 culturomics
K1 endocrine disruptors
K1 obesity
K1 obesogens
AB Integrated data from molecular and improved culturomics studies might offer holistic insights on gut microbiome dysbiosis triggered by xenobiotics, such as obesity and metabolic disorders. Bisphenol A (BPA), a dietary xenobiotic obesogen, was chosen for a directed culturing approach using microbiota specimens from 46 children with obesity and normal-weight profiles. In parallel, a complementary molecular analysis was carried out to estimate the BPA metabolising capacities. Firstly, catalogues of 237 BPA directed-cultured microorganisms were isolated using five selected media and several BPA treatments and conditions. Taxa from Firmicutes, Proteobacteria, and Actinobacteria were the most abundant in normal-weight and overweight/obese children, with species belonging to the genera Enterococcus, Escherichia, Staphylococcus, Bacillus, and Clostridium. Secondly, the representative isolated taxa from normal-weight vs. overweight/obese were grouped as BPA biodegrader, tolerant, or resistant bacteria, according to the presence of genes encoding BPA enzymes in their whole genome sequences. Remarkably, the presence of sporobiota and concretely Bacillus spp. showed the higher BPA biodegradation potential in overweight/obese group compared to normal-weight, which could drive a relevant role in obesity and metabolic dysbiosis triggered by these xenobiotics.
PB MDPI AG
YR 2022
FD 2022-01-03
LK http://hdl.handle.net/10668/21451
UL http://hdl.handle.net/10668/21451
LA en
NO López-Moreno A, Ruiz-Moreno Á, Pardo-Cacho J, Cerk K, Torres-Sánchez A, Ortiz P, et al. Culturing and Molecular Approaches for Identifying Microbiota Taxa Impacting Children's Obesogenic Phenotypes Related to Xenobiotic Dietary Exposure. Nutrients. 2022 Jan 6;14(2):241.
NO A.L.-M. has a Ph.D. contract through the EFSA grant and the programme “Intensificación de la Investigación” University of Granada (2019–2022). PO is under the contract “Garantía Juvenil” –FEDER-Junta de Andalucía. K. Cerk is under the EU-FORA Fellowship Programme. A.L-M., A.R-M., P.O., A.T.-S. and M.A. are part of the BIO-190 Research Group. They are also part of “UGR Plan Propio de Investigación 2019–2022. This work was carried out within the frame of FEDER-Infrastructure: IE19_198 UGR and OBEMIRISK EFSA-Partnering Grant Project GP/EFSA/ENCO/2018/03-GA04.
DS RISalud
RD Apr 19, 2025