TY - JOUR AU - Ruiz-Villalba, Adrián AU - Ruijter, Jan M. AU - van den Hoff, Maurice J. B. PY - 2021 DO - 10.3390/life11060496 UR - http://hdl.handle.net/10668/3514 T2 - Life AB - In the analysis of quantitative PCR (qPCR) data, the quantification cycle (Cq) indicates the position of the amplification curve with respect to the cycle axis. Because Cq is directly related to the starting concentration of the target, and the... LA - en PB - MDPI KW - qPCR analysis KW - Cq KW - Quantification cycle KW - Quantification threshold KW - PCR efficiency KW - Poisson variation KW - LOD KW - LOQ KW - Artefactos KW - Laboratorios KW - Expresión génica KW - Reacción en cadena en tiempo real de la polimerasa KW - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Artifacts KW - Medical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Facilities::Laboratories KW - Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression KW - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Real-Time Polymerase Chain Reaction TI - Use and Misuse of Cq in qPCR Data Analysis and Reporting TY - review article ER -