RT Journal Article
T1 Gene expression profiling in the intestinal mucosa of obese rats administered probiotic bacteria.
A1 Plaza-Diaz, Julio
A1 Robles-Sanchez, Candido
A1 Abadia-Molina, Francisco
A1 Saez-Lara, Maria Jose
A1 Vilchez-Padial, Laura Maria
A1 Gil, Angel
A1 Gomez-Llorente, Carolina
A1 Fontana, Luis
K1 Lacticaseibacillus rhamnosus
K1 Bifidobacterium breve
K1 Lacticaseibacillus paracasei
K1 Oligonucleotide Array Sequence Analysis
K1 RNA
K1 Administration, Oral
AB We investigated whether the administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 modulate the expression of genes in the intestinal mucosa of obese Zucker rats. Forty-eight Zucker-Leprfa/fa and 16 Zucker lean Lepr+/fa rats were used. Eight Zucker lean Lepr+/fa and 8 Zucker-Leprfa/fa rats were euthanized as a reference. The remaining 40 Zucker-Leprfa/fa rats were then assigned to receive 1010 colony forming units (CFU) of one of the three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo by oral administration for 30 days. An additional group of 8 Zucker lean Lepr+/fa rats received the placebo for 30 days. Over 27,000 rat genes were studied using a DNA array. Four animals per group were used. Total RNA was extracted from intestinal mucosa and cDNA was synthesized, fragmented and labeled. Labeled cDNA was hybridized using GeneChip kits, and the latter were scanned. Intensity values of each probe were processed and normalized to obtain an individual value for each set of probes.
PB Nature Publishing Group
YR 2017
FD 2017-10-27
LK http://hdl.handle.net/10668/11896
UL http://hdl.handle.net/10668/11896
LA en
NO Plaza-Díaz J, Robles-Sánchez C, Abadía-Molina F, Sáez-Lara MJ, Vilchez-Padial LM, Gil Á, et al. Gene expression profiling in the intestinal mucosa of obese rats administered probiotic bacteria. Sci Data. 2017 Dec 12;4:170186.
NO This paper will be part of Candido Robles-Sanchez’s doctorate, which is being completed as part of the‘Biochemistry and Molecular Biology Program’ at the University of Granada, Spain. This work wasfunded by the company Hero Spain, S. A. (grant #3545 managed by Fundación General EmpresaUniversidad de Granada) and by CEIBiotic, University of Granada, Spain (grant CEI2013P-11).
DS RISalud
RD Apr 5, 2025