RT Journal Article
T1 A CDK-regulated chromatin segregase promoting chromosome replication.
A1 Chacin, Erika
A1 Bansal, Priyanka
A1 Reusswig, Karl-Uwe
A1 Diaz-Santin, Luis M
A1 Ortega, Pedro
A1 Vizjak, Petra
A1 Gómez-González, Belen
A1 Müller-Planitz, Felix
A1 Aguilera, Andrés
A1 Pfander, Boris
A1 Cheung, Alan C M
A1 Kurat, Christoph F
AB The replication of chromosomes during S phase is critical for cellular and organismal function. Replicative stress can result in genome instability, which is a major driver of cancer. Yet how chromatin is made accessible during eukaryotic DNA synthesis is poorly understood. Here, we report the characterization of a chromatin remodeling enzyme-Yta7-entirely distinct from classical SNF2-ATPase family remodelers. Yta7 is a AAA+ -ATPase that assembles into ~1 MDa hexameric complexes capable of segregating histones from DNA. The Yta7 chromatin segregase promotes chromosome replication both in vivo and in vitro. Biochemical reconstitution experiments using purified proteins revealed that the enzymatic activity of Yta7 is regulated by S phase-forms of Cyclin-Dependent Kinase (S-CDK). S-CDK phosphorylation stimulates ATP hydrolysis by Yta7, promoting nucleosome disassembly and chromatin replication. Our results present a mechanism for how cells orchestrate chromatin dynamics in co-ordination with the cell cycle machinery to promote genome duplication during S phase.
YR 2021
FD 2021-09-01
LK http://hdl.handle.net/10668/18489
UL http://hdl.handle.net/10668/18489
LA en
DS RISalud
RD Apr 18, 2025